PMID: 8950315Jan 1, 1996Paper

Vesamicol, an inhibitor of acetylcholine vesicle packaging, increases synaptophysin phosphorylation in rat cortical synaptosomes

Life Sciences
K E Asermely, J J O'Neill

Abstract

Vesamicol (AH5183) is an inhibitor (IC50, 50 nM) of acetylcholine (ACh) vesicle packaging. Vesamicol increases the phosphorylation pattern of synaptophysin (p38), identified as a vesicle-specific phosphoprotein involved in vesicle-mediated neurotransmitter release. Percoll fractionation of the rat cortex yielded a cholinergic-enriched synaptosomal Fraction 4. Fraction 4 contained the highest enrichment of cholineacetyl-transferase activity (86 +/- 4.6 mumole AcCh/g protein/hr.) in the Percoll gradient. Fraction 4 demonstrated oxygen consumption (108 +/- 23.4 nmole/mg protein), levels of adenosine triphosphate, ATP, (10.29 +/- 0.45 nmole/mg protein) and adenosine diphosphate, ADP, (10.54 +/- 2.72 nmole/mg protein), energy potential (ATP/[ADP] [Pi], (0.49) phosphate uptake (65-80 nmoles phosphate/mg tissue), 32Pi labelling (130 +/- 12 x 10(5) DPM/mg tissue; 74 +/- 9.8 x 10(2) nmoles phosphate/mg tissue). Synaptophysin was identified by Western blotting and confirmed by qualitative immunoprecipitation. Synaptophysin phosphorylation was confirmed by autoradiograph. Synaptophysin phosphorylation increased (225%) in the presence of vesamicol (ED50, 1 nM) in Fraction 4. Vesamicol (50 nM) and vanadate (54 microM) were compared for thei...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Dec 1, 1990·The Journal of Allergy and Clinical Immunology·W A Massey, L M Lichtenstein
Apr 30, 1986·Brain Research·P R DunkleyJ A Rostas
Nov 25, 1987·Nucleic Acids Research·T C SüdhofR Jahn
Dec 1, 1987·The Journal of Cell Biology·K M BuckleyR B Kelly
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D T PangP Greengard
Jan 1, 1986·Progress in Brain Research·P R Dunkley, P J Robinson
Jun 1, 1987·Neurochemical Research·J J O'Neill, K A Hruschak
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R JahnP Greengard
May 1, 1966·Journal of Biochemistry·K OzawaC Araki
May 1, 1970·British Journal of Pharmacology·I G Marshall
Aug 1, 1984·Biochemical Society Transactions·V P Whittaker
Jan 1, 1981·Current Topics in Cellular Regulation·T Ramasarma, F L Crane
Mar 1, 1981·Journal of Neurochemistry·D E GmerekJ J O'Neill
Dec 1, 1981·Journal of Neurochemistry·A SalaminR W Straub
Feb 1, 1995·The Biochemical Journal·P WashbourneC Montecucco
Feb 1, 1993·Synapse·J L RubensteinA J Czernik
Jun 18, 1958·The Journal of Physiology·C O HEBB, V P WHITTAKER
Apr 1, 1965·Journal of Neurochemistry·R E MCCAMAN, J M HUNT
Jan 1, 1986·Neurochemistry International·P J Robinson, W Lovenberg

❮ Previous
Next ❯

Citations

Oct 18, 2008·Nature Protocols·Peter R DunkleyPhillip J Robinson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basal Forebrain- Circuits

Basal forebrain is a region in the brain important for production of acetylcholine and is the major cholinergic output of the CNS. Discover the latest research on circuits in the basal forebrain here.