Vesicle-associated membrane protein 2 plays a specific role in the insulin-dependent trafficking of the facilitative glucose transporter GLUT4 in 3T3-L1 adipocytes.

The Journal of Biological Chemistry
L B MartinDavid E James

Abstract

Vesicle-associated membrane protein 2 (VAMP2) has been implicated in the insulin-regulated trafficking of GLUT4 in adipocytes. It has been proposed that VAMP2 co-localizes with GLUT4 in a postendocytic storage compartment (Martin, S., Tellam, J., Livingstone, C., Slot, J. W., Gould, G. W., and James, D. E. (1996) J. Cell Biol. 134, 625-635), suggesting that it may play a role distinct from endosomal v-SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) such as cellubrevin that are also expressed in adipocytes. The present study examines the effects of recombinant glutathione S-transferase (GST) fusion proteins encompassing the entire cytoplasmic tails of VAMP1, VAMP2, and cellubrevin on insulin-stimulated GLUT4 translocation in streptolysin O permeabilized 3T3-L1 adipocytes. GST-VAMP2 inhibited insulin-stimulated GLUT4 translocation by approximately 35%, whereas GST-VAMP1 and GST-cellubrevin were without effect. A synthetic peptide corresponding to the unique N terminus of VAMP2 also inhibited insulin-stimulated GLUT4 translocation in a dose-dependent manner. This peptide had no effect on either guanosine 5'-3-O-(thio)triphosphate-stimulated GLUT4 translocation or on insulin-stimulated GLUT1 transloc...Continue Reading

References

Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J W SlotG E Lienhard
Mar 1, 1991·The American Journal of Physiology·R C PiperD E James
Nov 1, 1984·Infection and Immunity·S BhakdiJ Tranum-Jensen
Feb 1, 1995·The Biochemical Journal·P WashbourneC Montecucco
Apr 29, 1994·Biochemical and Biophysical Research Communications·S YamasakiH Niemann
Aug 16, 1994·Proceedings of the National Academy of Sciences of the United States of America·K V Kandror, P F Pilch
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M K Bennett, R H Scheller
Nov 1, 1993·Cellular Signalling·N W MerrallG W Gould
Sep 1, 1993·Medical Microbiology and Immunology·S BhakdiM Palmer
Mar 25, 1993·Nature·T SöllnerJ E Rothman
Feb 9, 1996·The Journal of Biological Chemistry·S A RossS R Keller
Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·B CheathamA Klip
May 1, 1994·Trends in Cell Biology·H NiemannR Jahn
Apr 1, 1994·Trends in Cell Biology·D E JamesJ W Slot

❮ Previous
Next ❯

Citations

Oct 25, 2002·Diabetes/metabolism Research and Reviews·Silvia Mora, Jeffrey E Pessin
Apr 25, 2000·Biochemical and Biophysical Research Communications·G R HicksonG W Gould
Jun 8, 2001·Biochemical and Biophysical Research Communications·E TomàsA Zorzano
May 7, 2011·Cellular and Molecular Life Sciences : CMLS·Laura K M SteinbuschJoost J F P Luiken
Mar 31, 2004·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Xiujun Zhang, Gale B Carey
Apr 18, 2001·Trends in Cell Biology·G D Holman, I V Sandoval
May 24, 2012·Nature Reviews. Molecular Cell Biology·Dara Leto, Alan R Saltiel
Nov 1, 2011·Molecular Biology of the Cell·Meritxell ReverterCarlos Enrich
Jul 30, 2004·Molecular and Cellular Biology·Danielle N GrossPaul F Pilch
Aug 18, 2004·Molecular and Cellular Biology·Makoto FunakiPaul A Janmey
Jan 8, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jenna L JewellDebbie C Thurmond
May 26, 2001·The Journal of Clinical Investigation·C YangJ E Pessin
May 26, 2001·The Journal of Clinical Investigation·M Mueckler
Jan 17, 2012·Journal of Cell Science·Jacqueline StöckliDavid E James
Jan 30, 2008·The Journal of Cell Biology·Dumaine Williams, Jeffrey E Pessin
Sep 11, 2007·FEBS Letters·Miki OkayamaTaishin Takuma
Jul 17, 2003·Acta Physiologica Scandinavica·A Rudich, A Klip
Apr 17, 2004·Traffic·Mhairi C TowlerFrances M Brodsky
Dec 2, 2005·EMBO Reports·Chandrasagar B Dugani, Amira Klip
May 4, 2005·Molecular and Cellular Endocrinology·Mathias UhligJürgen Eckel
Apr 4, 2007·Cell Metabolism·Shaohui Huang, Michael P Czech
Dec 17, 2014·Molecular Biology of the Cell·Jessica B A SadlerGwyn W Gould

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.