Vesicle fluctuation analysis of the effects of sterols on membrane bending rigidity

European Biophysics Journal : EBJ
J R HenriksenJ H Ipsen

Abstract

Sterols are regulators of both biological function and structure. The role of cholesterol in promoting the structural and mechanical stability of membranes is widely recognized. Knowledge of how the related sterols, lanosterol and ergosterol, affect membrane mechanical properties is sparse. This paper presents a comprehensive comparison of the effects of cholesterol, lanosterol, and ergosterol upon the bending elastic properties of 1-palmitoyl-2-oleoyl- sn-glycero-3-phosphocholine giant unilamellar vesicles. Measurements are made using vesicle fluctuation analysis, a nonintrusive technique that we have recently improved for determining membrane bending rigidity. Giving a detailed account of the vesicle fluctuation analysis technique, we describe how the gravitational stabilization of the vesicles enhances image contrast, vesicle yield, and the quality of data. Implications of gravity on vesicle behaviour are also discussed. These recent modifications render vesicle fluctuation analysis an efficient and accurate method for determining how cholesterol, lanosterol, and ergosterol increase membrane bending rigidity.

References

Nov 27, 1987·Biochimica Et Biophysica Acta·J H IpsenM J Zuckermann
Nov 1, 1973·Zeitschrift Für Naturforschung. Teil C: Biochemie, Biophysik, Biologie, Virologie·W Helfrich
Jan 1, 1983·CRC Critical Reviews in Biochemistry·K E Bloch
Nov 1, 1987·Physical Review A: General Physics·S T Milner, S A Safran
Apr 23, 1990·Physical Review Letters·E Evans, W Rawicz
Jul 30, 2003·Biophysical Journal·Philipus J Patty, Barbara J Frisken
Mar 11, 2004·The European Physical Journal. E, Soft Matter·J R Henriksen, J H Ipsen
Mar 12, 2004·The European Physical Journal. E, Soft Matter·L MiaoJ Kleis
Jul 16, 2004·The European Physical Journal. E, Soft Matter·J R Henriksen, J H Ipsen

❮ Previous
Next ❯

Citations

Feb 25, 2005·Lipids·Ole G Mouritsen, Martin J Zuckermann
May 26, 2011·Cold Spring Harbor Perspectives in Biology·Ole G Mouritsen
Nov 1, 2011·The European Physical Journal. E, Soft Matter·P MéléardJ H Ipsen
Feb 27, 2007·PLoS Computational Biology·Perttu S NiemeläIlpo Vattulainen
Jul 23, 2013·PloS One·Iván Rey SuárezAndres Gonzalez-Mancera
Jun 14, 2013·The Journal of Chemical Physics·Mingyang HuMarkus Deserno
Nov 21, 2007·The Journal of Cell Biology·Anna V ShnyrovaVadim A Frolov
May 30, 2009·Chemistry and Physics of Lipids·Olav GarvikDaniel Wüstner
Jul 8, 2008·Biophysical Chemistry·Hélène BouvraisJohn H Ipsen
May 28, 2014·Biochimica Et Biophysica Acta·Luka KristancGregor Gomišček
May 29, 2014·Biochimica Et Biophysica Acta·Tripta BhatiaAdam Cohen Simonsen
Aug 26, 2015·Scientific Reports·Angelo PommellaValeria Garbin
Mar 25, 2008·Biochimica Et Biophysica Acta·Rohit Sood, Paavo K J Kinnunen
Dec 6, 2005·Biophysical Journal·J HenriksenJ H Ipsen
Jun 12, 2013·Biophysical Journal·Amit ChoubeyPriya Vashishta
Jul 27, 2011·Biochimica Et Biophysica Acta·Robert BurmanUlf Göransson
Sep 16, 2009·Biophysical Journal·Aiwei TianTobias Baumgart
Feb 11, 2015·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Karina Vincents FiskerMartin Holmstrup
Jun 3, 2008·Biophysical Journal·Karen SabatiniPaavo K J Kinnunen
Apr 27, 2012·Chemistry and Physics of Lipids·Christiane DeckerSylvio May
Jul 24, 2007·Biophysical Journal·Cinzia EspositoTobias Baumgart
Mar 30, 2012·Biophysical Journal·Mingyang HuMarkus Deserno
Mar 29, 2014·Advances in Colloid and Interface Science·Rumiana Dimova
Jul 5, 2011·Biophysical Journal·Chad LeidyGünther H Peters
May 21, 2010·Biophysical Journal·Jonas R HenriksenJohn H Ipsen
Apr 20, 2010·Biochimica Et Biophysica Acta·Hélène BouvraisPhilippe Méléard
Apr 14, 2016·The Journal of Physical Chemistry. B·Xin Wang, Markus Deserno
May 20, 2014·Chemistry and Physics of Lipids·Markus Deserno
Nov 3, 2016·Biophysical Journal·Nico Fricke, Rumiana Dimova
Apr 4, 2017·Chemistry and Physics of Lipids·Ole G MouritsenAdam Cohen Simonsen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.