Vesicles containing Chlamydia trachomatis serovar L2 remain above pH 6 within HEC-1B cells.

Infection and Immunity
N SchrammP B Wyrick

Abstract

Chlamydia trachomatis serovar L2 is an obligate intracellular bacterium which is internalized in target epithelial cells by endocytosis and resides within a membrane-bound vesicle. Over the next several hours following entry, individual serovar L2-containing vesicles fuse with one another to form a single membrane-bound vesicle (or inclusion) within which the microcolony develops. The experiments reported here directly examined the pH of vesicles containing chlamydiae. The pH was determined by measuring emission ratios of the fluorescent, pH-sensitive probe SNAFL (5-[and 6-]-carboxyseminaphthofluorescein-1, succinimidyl ester) conjugated to chlamydiae. The pH remained above 6.0 at 2, 4, and 12 h after infection, while the pH of vesicles contained heat-killed organisms fell 5.3. In the presence of amines, which raise the pH of acidic compartments, C. trachomatis inclusion formation was unaffected. Inactivation of Na+,K+ -ATPases, the ion pumps responsible for maintaining a pH above 6 within early endocytic vesicles, inhibited the growth of C. trachomatis within epithelial cells. Preventing vesicular acidification by inhibiting the vacuolar proton ATPase did not affect chlamydial growth. Thus, chlamydiae do not reside within high...Continue Reading

References

Mar 1, 1978·Infection and Immunity·P B Wyrick, E A Brownridge
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C M Alpuche ArandaS I Miller
May 1, 1991·Analytical Biochemistry·J E WhitakerF G Prendergast
Mar 8, 1991·Cell·J P GorvelJ Gruenberg
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D TranL Orci
Dec 1, 1987·The Journal of Cell Biology·D J Yamashiro, F R Maxfield
Jun 1, 1989·The Journal of Cell Biology·B M MullockJ P Luzio
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R FuchsI Mellman
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·C C CainR F Murphy
Aug 1, 1989·Infection and Immunity·P B WyrickC R Bagnell
May 5, 1985·Nature·L D SibleyJ L Krahenbuhl
Jun 1, 1988·Infection and Immunity·R L HodinkaP B Wyrick
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R G AndersonM S Brown
Dec 1, 1984·The Journal of Cell Biology·M A Horwitz, F R Maxfield
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T Hackstadt, J C Williams
Sep 1, 1994·The Journal of Cell Biology·B M MullockJ P Luzio
Jan 1, 1995·Infection and Immunity·N Schramm, P B Wyrick
Jan 1, 1994·Methods in Enzymology·T H Steinberg, J A Swanson
Jan 1, 1994·Methods in Enzymology·J Schachter, P B Wyrick
Jun 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·K W DunnF R Maxfield
Jun 1, 1993·The Journal of Experimental Medicine·L G EissenbergP H Schlesinger

❮ Previous
Next ❯

Citations

Apr 18, 1998·The Journal of Biological Chemistry·D M OjciusA Dautry-Varsat
Feb 24, 2001·Cellular Microbiology·P B Wyrick
Aug 19, 2007·Journal of Bacteriology·Teresa N Giles, David E Graham
Sep 2, 2008·Applied and Environmental Microbiology·Junji MatsuoHiroyuki Yamaguchi
Mar 17, 2011·PloS One·Scot P OuelletteRey A Carabeo
Nov 9, 2012·Critical Reviews in Microbiology·Delphine S BeeckmanDaisy Vanrompay
Oct 10, 2012·FEMS Microbiology Letters·Kimberly A BlivenAnthony T Maurelli
Oct 6, 2000·Annual Review of Microbiology·M S Swanson, B K Hammer
Jan 1, 1997·Annual Review of Microbiology·A P Sinai, K A Joiner
Sep 19, 2000·Infection and Immunity·N MohagheghpourL E Bermudez
Dec 31, 2005·BioTechniques·Anton BeletskiiLiming Yu
May 14, 2003·Proceedings of the National Academy of Sciences of the United States of America·Reynaldo A CarabeoTed Hackstadt
Aug 3, 2011·Infection and Immunity·Muhammad YasirHuizhou Fan
Jul 27, 2002·Annual Review of Cell and Developmental Biology·Kenneth A Fields, Ted Hackstadt
Jan 30, 2018·Frontiers in Cellular and Infection Microbiology·Rafat ZrieqJohannes H Hegemann
Aug 21, 2020·Infection and Immunity·Svea MatthiesenMichael R Knittler
Mar 12, 2021·American Journal of Obstetrics and Gynecology·B Todd ChappellBrandon Howard
Feb 4, 1998·Analytical Biochemistry·I VergneG Lanéelle
Mar 6, 1999·Current Opinion in Microbiology·T Hackstadt
Mar 14, 2003·International Journal for Parasitology·Walter K Andreoli, Renato A Mortara
Nov 23, 2007·Genome Research·Nicholas R ThomsonIan N Clarke

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.