Vesicular phospholipid gel-based depot formulations for pharmaceutical proteins: development and in vitro evaluation

Journal of Controlled Release : Official Journal of the Controlled Release Society
W TianG Winter

Abstract

The object of this study was to evaluate the potential of vesicular phospholipid gels (VPGs) as an alternative delivery system for therapeutic proteins. Therefore, the model protein erythropoietin (EPO) was incorporated into various VPG formulations by dual asymmetric centrifugation. In order to characterize and to quantify the incorporated protein, different extraction protocols were investigated. Among several detergents and organic solvents an extraction method applying chloroform was found most suitable. SDS-PAGE analysis of EPO extracted from VPGs revealed excellent protein stability which was maintained in the VPGs' matrix after an incorporation process with dual asymmetric centrifuge. Irrespective of the investigated formulation all VPGs delivered the protein over prolonged periods of time at close to linear kinetics without any burst effect. Both the lipid content and the lipid charge had a strong influence on the release behavior. For instance formulations based on 300 mg/g lipid delivered 83% EPO after 280 h while gels based on 550 mg/g lipid liberated 64% within 400 h. From the parallelism of release and erosion kinetics found for all formulations it was concluded that erosion rather than diffusion is the dominant re...Continue Reading

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Citations

Nov 13, 2015·Drug Development and Industrial Pharmacy·Yu ZhangZhirong Zhang
Jan 9, 2015·Pharmaceutical Development and Technology·Muhammad Sajid Hamid AkashShuqing Chen
Jan 1, 2013·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Claudia KutzaKarsten Mäder
Sep 12, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Gerhard SaxGerhard Winter
Feb 22, 2012·International Journal of Pharmaceutics·Na QiYu Zhang
Mar 5, 2013·International Journal of Pharmaceutics·Frederike TenambergenKarsten Mäder
Feb 18, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yosra S R ElnaggarOssama Y Abdallah
Oct 18, 2016·Annals of the New York Academy of Sciences·Sahishnu PatelWilliam N Levine
May 17, 2017·International Journal of Pharmaceutics·Sveinung G IngebrigtsenAnn Mari Holsæter
Oct 23, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Shirin TavakoliArto Urtti

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