Vesicular stomatitis virus glycoprotein: a transducing coat for SFV-based RNA vectors

The Journal of Gene Medicine
Fabien DorangeJean-Christophe Pagès

Abstract

Semliki Forest virus (SFV) vectors have a great potential for the induction of protective immunity in a large number of clinical conditions including cancer. Such a potential accounts for the huge efforts made to improve the in vivo expression from SFV vectors. It is noteworthy that efficient in vivo expression strongly relies on the ability to deliver high-titre vectors. To achieve this, the generation of recombinant SFV particles, using independent expression systems for structural SFV genes, has been proposed. However, despite several modifications in the production process, a risk of contamination with replication-competent, or partially recombined, virus has remained. Here, we exploit the ability of the vesicular stomatitis virus glycoprotein (VSV-G), expressed in trans, to hijack full-length genomic SFV RNA into secreted virus-like particles (VLPs). To allow SFV vector mobilisation, we designed a CMV driven SFV vector in which the internal 26S promoter has been extensively mutated. With this vector, mobilisation events were monitored using the Green Fluorescent Protein (GFP). The production procedure involves a sequential transfection protocol, of plasmids expressing the VSV-G and the SFV vector respectively. We show that...Continue Reading

Citations

Jul 14, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Philippe-Emmanuel MangeotVincent Lotteau
Oct 19, 2005·Gene Therapy·E PiverJ-C Pagès
Sep 16, 2006·Journal of Virology·Eric PiverJean-Christophe Pagès
Apr 17, 2013·BMC Biotechnology·Anne PrelJean-Christophe Pagès
Nov 10, 2016·Vaccines·Kenneth Lundstrom
Nov 4, 2015·Molecular Therapy. Methods & Clinical Development·Anne PrelJean-Christophe Pagès

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