Vessel painting of the microcirculation using fluorescent lipophilic tracers

Microvascular Research
Dino J RavnicMoritz A Konerding

Abstract

Flexible approaches to defining microvessel morphometry are useful in the study of both acute and chronic structural changes of the microcirculation. In this report, we examined the utility of the intravascular infusion of lipophilic carbocyanine tracers in the structural assessment of the retina, skin, lung, and colon microcirculation. The microvessel labeling technique, here termed fluorescent vessel painting, involved the intravascular injection of sulfonated lipophilic carbocyanine tracers. The utility of vessel painting in morphometry was assessed using morphometric comparisons with corrosion casting and 2-dimensional and 3-dimensional scanning electron microscopy. The comparisons demonstrated that fluorescent vessel painting modestly overestimated the interbranch angles, interbranch distances, and vessel diameters of the 2D mucosal plexus of the colon. These differences were narrowed with the application of confocal microscopy. The advantages of fluorescence vessel painting included (1) the filling of all tissues including the relatively high resistance microvessels of the mouse skin, (2) the ability to use tissue counterstains such as DAPI, and (3) the prolonged stability of the lipophilic tracer after aldehyde fixation....Continue Reading

References

Jul 13, 1989·Nature·P K Horan, S E Slezak
Jan 1, 1989·Cellular Immunology·M J MelnicoffP S Morahan
Apr 1, 1995·In Vitro Cellular & Developmental Biology. Animal·K AbdiS J Mentzer
Mar 22, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C A WestS J Mentzer
May 4, 2001·Journal of Immunological Methods·M SuS J Mentzer
Jun 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Timothy W SecombSteven J Mentzer

❮ Previous
Next ❯

Citations

Jun 27, 2013·Diabetologia·Shiue-Cheng TangYa-Yuan Fu
Sep 23, 2008·In Vitro Cellular & Developmental Biology. Animal·Aslihan TurhanSteven J Mentzer
Aug 23, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Min DaiXiaorui Shi
Jun 16, 2011·PloS One·Min Dai, Xiaorui Shi
Sep 1, 2009·Microvascular Research·Nenad FilipovicSteven J Mentzer
Sep 26, 2008·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·Thomas HolzbachRiccardo E Giunta
Jul 7, 2007·The Journal of Surgical Research·Dino J RavnicSteven J Mentzer
Apr 22, 2009·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Aslihan TurhanSteven J Mentzer
Aug 1, 2009·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Lino F MieleSteven J Mentzer
Nov 23, 2006·Journal of Anatomy·Dino J RavnicSteven J Mentzer
Oct 22, 2008·Microscopy Research and Technique·Grace S LeeSteven J Mentzer
Mar 15, 2013·Microvascular Research·Maximilian AckermannMoritz A Konerding
Jun 25, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·Pei-Yu LinShiue-Cheng Tang
Dec 19, 2007·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Aslihan TurhanSteven J Mentzer
Mar 5, 2019·Expert Opinion on Drug Discovery·Ben Waldau
Jan 28, 2014·Biomedical Optics Express·Austin J MoyBernard Choi
Jan 16, 2014·American Journal of Physiology. Endocrinology and Metabolism·Jyuhn-Huarng JuangShiue-Cheng Tang
Oct 23, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ya-Yuan FuShiue-Cheng Tang
Dec 15, 2020·Microvascular Research·Patrick C HancockDino J Ravnic

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.