PMID: 9190808Jun 1, 1997Paper

Vfr controls quorum sensing in Pseudomonas aeruginosa

Journal of Bacteriology
A M AlbusB H Iglewski

Abstract

Pseudomonas aeruginosa controls several genes in a cell density-dependent manner through a phenomenon termed quorum sensing. The transcriptional activator protein of the las quorum-sensing system is encoded for by the lasR gene, which is at the top of a quorum-sensing hierarchy. The activation of LasR as a transcriptional activator induces the expression of multiple genes that code for factors important for virulence, and rhlR, which encodes the transcriptional activator protein of the P. aeruginosa rhl quorum-sensing system. Elucidating the method of lasR regulation is crucial to understanding P. aeruginosa quorum sensing. In this report, we present studies on the transcriptional control of lasR. We identified two distinct transcriptional start sites for lasR that were located 201 bp (transcript T1) and 231 bp (transcript T2) upstream from the lasR start of translation. With the use of transcriptional lasRp-lacZ fusions, we showed that in P. aeruginosa, lasR expression is cell density dependent. This gene was expressed at a basal level until it was induced during the second half of log-phase growth, with expression becoming maximal during stationary-phase growth. We also showed that lasR expression was regulated through the cy...Continue Reading

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Citations

Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·M R Parsek, E P Greenberg
Nov 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·H CaoL G Rahme
Apr 10, 2004·Microbiology·Michael HogardtJürgen Heesemann
Apr 9, 2005·Environmental Microbiology·Mario JuhasBurkhard Tümmler
Oct 22, 2005·FEMS Microbiology Letters·Haijin XuMingqiang Qiao
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Oct 10, 2006·Cellular Microbiology·Mary Jo Kirisits, Matthew R Parsek
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Jun 15, 2011·Applied Microbiology and Biotechnology·Markus Michael Müller, Rudolf Hausmann
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Mar 29, 2018·BMC Genomics·Erin E GillFiona S L Brinkman

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