VGAT and VGLUT2 expression in MCH and orexin neurons in double transgenic reporter mice

IBRO Reports
Carlos Blanco-CenturionMeng Liu

Abstract

The neuropeptides orexin and melanin-concentrating hormone (MCH), as well as the neurotransmitters GABA (γ-Aminobutyric acid) and glutamate are chief modulators of the sleep-wake states in the posterior hypothalamus. To investigate co-expression of vesicular GABA transporter (VGAT, a marker of GABA neurons) and the vesicular glutamate transporter-2 (VGLUT2, a marker of glutamate neurons) in orexin and MCH neurons, we generated two transgenic mouse lines. One line selectively expressed the reporter gene EYFP in VGAT+ neurons, whereas the other line expressed reporter gene tdTomato in VGLUT2+ neurons. Co-localization between these genetic reporters and orexin or MCH immunofluorescent tags was determined using 3D computer reconstructions of Z stacks that were acquired using a multiphoton laser confocal microscope. Our results demonstrated that MCH neurons expressed neither VGAT nor VGLUT2, suggesting MCH neurons are a separate cluster of cells from VGAT+ GABAergic neurons and VGLUT2+ glutamatergic neurons. Moreover, most orexin neurons expressed VGLUT2, indicating these neurons are glutamatergic. Our data suggested that in the posterior hypothalamus there are four major distinct groups of neurons: VGAT+, orexin+/VGLUT2+, orexin-/V...Continue Reading

Citations

Nov 1, 2018·Molecular Psychiatry·Jun OhLea T Grinberg
Aug 28, 2020·The Journal of Comparative Neurology·Paula G F QuaresmaJose Donato
Oct 16, 2018·Frontiers in Pharmacology·Idris A AzeezMarina Bentivoglio
Oct 22, 2019·European Journal of Immunology·Andrea CossarizzaArturo Zychlinsky
Jun 29, 2021·Journal of Chemical Neuroanatomy·Xunlei ZhouGonzalo Alvarez-Bolado
Jul 6, 2021·Metabolic Brain Disease·Idris A AzeezJames O Olopade

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