VH and VL region structure of antibodies that recognize the (NANP)3 dodecapeptide sequence in the circumsporozoite protein of Plasmodium falciparum

European Journal of Immunology
R AnkerB A Pollok

Abstract

The sporozoite form of Plasmodium falciparum displays on its surface the circumsporozoite (CS) protein. The central domain of this protein possesses a reiterated tetrapeptide sequence Asn-Ala-Asn-Pro (NANP), and greater than 90% of the sporozoite-specific antibodies obtained from individuals living in malaria endemic areas recognize epitopes within this repeat sequence. Considering the highly repetitive structure of this naturally occurring antigen and its immunodominance, we were interested in analyzing the structural diversity of antibodies that bind to the (NANP)3 sequence. Molecular characterization of immunoglobulin heavy and light chain mRNA was performed for five hybridomas that produce antibodies with binding specificity for the dodecapeptide (NANP)3. These hybridomas were produced in BALB/c mice by inoculation with whole P. falciparum sporozoites. Sequence analysis and Northern blotting showed that for heavy chain, three hybridomas used VH elements that belong to the VHIX family and two to the VHJ558 family. Four different V kappa subgroups were represented among the light chains. Different D and J kappa segments are also utilized, while four heavy chain gene rearrangements involved the JH4 segment. These results indic...Continue Reading

References

Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E E MaxP Leder
Mar 1, 1979·Journal of Cellular Physiology·R P PerryC M Croce
Apr 1, 1990·European Journal of Immunology·D StüberH Matile
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M F GoodL H Miller
Oct 1, 1989·Immunology Today·U KrawinkelT Blankenstein
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J GeliebterS G Nathenson
Apr 14, 1983·Nature·S Tonegawa
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·W D CookM D Scharff
Jul 1, 1982·The Journal of Experimental Medicine·E H NardinY Chomcharn

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Citations

May 1, 1993·The Journal of Experimental Medicine·C MohanS K Datta
Aug 13, 2014·Proceedings of the National Academy of Sciences of the United States of America·Cailin DealGary Ketner
Mar 10, 2017·Expert Review of Vaccines·Brandon SackD Noah Sather

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