VH gene analysis in sporadic Burkitt's lymphoma: somatic mutation and intraclonal diversity with special reference to the tumor cells involving germinal center

Leukemia & Lymphoma
Kouichi IsobeH Ito

Abstract

We analyzed the immunoglobulin heavy chain variable region (V(H)) gene in seven cases of sporadic Burkitt's lymphoma (sBL) to elucidate their cell of origin. In particular, we focused on the V(H) gene status of tumor cells involving adjacent germinal center (GC) by microdissecting histological sections. Among the seven V(H) genes V(H)1 family was found in two, V(H)3 in four, and V(H)4 in one. All rearranged V(H) genes demonstrated somatic mutations at percentages ranging from 1.4 to 7.5% (mean, 4.2%), which is a similar level to that seen in IgM-only B cells. Three out of four V(H) genes with more than 2% sequence difference from their corresponding germline counterpart showed evidence of antigen selection in their framework region 3. Three cases demonstrated signs of intraclonal diversity with a mutational frequency of 0.47-0.98%, which was 13.5-28.8 times as great as the Taq infidelity in our experimental conditions. However the level of somatic mutation and the effect of antigen selection on V(H) gene were diverse in these three cases, and the relationship between V(H) gene somatic mutation status and intraclonal diversity was unclear in sBL. In the analysis of microdissected tissues, all 20 tumor clones in the adjacent GCs ...Continue Reading

References

Sep 23, 1976·The New England Journal of Medicine·R B MannC W Berard
Mar 1, 1995·The Journal of Clinical Investigation·Y W ChuR A Insel
Jul 1, 1994·Current Biology : CB·A EhlichK Rajewsky
Jan 1, 1997·British Journal of Haematology·M Q DuL X Pan
Jun 1, 1997·The Journal of Pathology·D H Wright
May 29, 1998·Immunological Reviews·U KleinR Küppers
Aug 26, 1998·Leukemia & Lymphoma·C J ChapmanF K Stevenson
Dec 16, 1998·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·C ThiedeA Neubauer

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Citations

Aug 22, 2008·Postgraduate Medical Journal·G BradyP J Farrell
Mar 1, 2003·Journal of Clinical Pathology·C BellanL Leoncini
Nov 22, 2011·PloS One·Volkhard SeitzMichael Hummel
May 21, 2008·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·M R Campanero

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