PMID: 9171896Dec 1, 1996Paper

VH repertoire in human B lymphocytes stimulated by CD40 ligand and IL-4: evidence for positive and negative selection mechanisms coupled to CD40 activation

Molecular Immunology
G V PinchukE C Milner

Abstract

In the normal immune system, B cells are thought to be negatively or positively selected at various checkpoints during their maturation; a process that maintains a broad immunoglobulin repertoire while eliminating non-functional or potentially harmful autoreactive antibodies. This study tested the hypothesis that utilization of certain immunoglobulin heavy chain variable region (VH) genes, possibly as a consequence of intrinsic affinity for various ligands, directs positive or negative B cell selection coupled to B cell activation in the periphery during the immune response. The specific prediction that the VH repertoire of CD40-activated B cells would differ from the repertoire of unstimulated cells from the same donor, was tested by assessing VH utilization among human B cell clones grown in vitro, following stimulation with CD40 ligand (CD40L) and IL-4. The results showed that, although utilization of the known VH families and of individual VH3 genes was similar to that found in unstimulated B lymphocytes of the same donor, utilization of individual VH4 genes in CD40-activated B cells displayed a pattern that was markedly different from that of the unstimulated B cells. An allele of V4-61, V4-61b, was over-represented among ...Continue Reading

References

Sep 1, 1990·Molecular Immunology·V GuigouF Fumoux
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D Nemazee, K Buerki
Jan 1, 1989·Cold Spring Harbor Symposia on Quantitative Biology·C C GoodnowM Loughnan
Apr 14, 1983·Nature·S Tonegawa
Nov 17, 1995·Science·T S GriffithT A Ferguson
Dec 1, 1993·Current Opinion in Immunology·R FuleihanR S Geha
Nov 1, 1995·The Journal of Experimental Medicine·P GarroneJ Banchereau
Feb 3, 1994·Nature·E A Clark, J A Ledbetter
Mar 19, 1996·Proceedings of the National Academy of Sciences of the United States of America·C C Goodnow
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·S K RayB Diamond

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Citations

Jul 5, 2005·Human Immunology·Tania Romo-GonzálezEnrique Vargas-Madrazo
Jun 16, 2009·Seminars in Immunology·Daphne Y Ma, Edward A Clark
Nov 23, 2006·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Masaaki MiyazawaHiroyuki Suzuki
Nov 7, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tâm D QuáchThomas L Rothstein
Aug 4, 2005·Blood·Kostas StamatopoulosAthanasios Fassas

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