Vhr1p, a new transcription factor from budding yeast, regulates biotin-dependent expression of VHT1 and BIO5.

The Journal of Biological Chemistry
Matthias WeiderN Sauer

Abstract

Transcription of the Saccharomyces cerevisiae vitamin H transporter gene VHT1 is enhanced by low extracellular biotin. Here we present the identification and characterization of Vhr1p as a transcriptional regulator of VHT1 (VHR1 (YIL056w); VHT1 regulator 1) and the identification of the cis-regulatory target sequences for Vhr1p in two yeast promoters. VHR1 was identified in a complementation screening of mutagenized yeast cells that had lost the capacity to express the gene of the green fluorescent protein (GFP) from the VHT1 promoter. Deltavhr1 deletion mutants fail to induce VHT1 on low biotin concentrations. In yeast one-hybrid analyses performed with fusions of Vhr1p N-terminal and C-terminal fragments to the Gal4p activation domain or to the Gal4p DNA-binding domain, the Vhr1p N terminus mediated biotin-dependent DNA binding, and the Vhr1p C terminus triggered biotin-dependent transcriptional activation. The analyzed Vhr1p N-terminal fragment has previously been described as a domain of unknown function (DUF352). Deletion and linker scanning analyses of the VHT1 promoter revealed the palindromic 18-nucleotide sequence AATCA-N8-TGAYT as the vitamin H-responsive element. This sequence was identified also in the BIO5 promoter...Continue Reading

References

Apr 2, 1992·Nature·R MarmorsteinS C Harrison
Mar 25, 1992·Nucleic Acids Research·D GietzR H Schiestl
Jan 1, 1991·Methods in Enzymology·F Sherman
Jan 1, 1989·Annual Review of Biochemistry·J R Knowles
Jan 1, 1988·Cold Spring Harbor Symposia on Quantitative Biology·D BotsteinR A Kahn
Mar 1, 1988·Molecular and Cellular Biology·S Michaelis, I Herskowitz
Feb 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P MadauleA M Myers
Nov 1, 1969·Journal of Bacteriology·T O Rogers, H C Lichstein
Nov 1, 1969·Journal of Bacteriology·T O Rogers, H C Lichstein
Aug 1, 1995·FEMS Microbiology Letters·J E Cronan, J C Wallace
Aug 1, 1994·Molecular and Cellular Biology·E Remboutsika, G B Kohlhaw
Feb 1, 1994·Protein Engineering·C Geourjon, G Deléage
Jul 20, 1993·Journal of Molecular Biology·B Rost, C Sander
Jan 1, 1993·Trends in Biochemical Sciences·M D Marger, M H Saier
Mar 1, 1996·Current Biology : CB·W ChiuJ Sheen
Jun 1, 1996·Methods : a Companion to Methods in Enzymology·M Malmqvist
Mar 1, 1997·Trends in Biochemical Sciences·J Svaren, W Hörz
May 1, 1997·Molecular and Cellular Biology·W V Ding, S A Johnston
Jun 1, 1997·Fungal Genetics and Biology : FG & B·R B Todd, A Andrianopoulos
Mar 7, 1998·The Biochemical Journal·H R Graack, B Wittmann-Liebold
Jul 4, 1998·The Journal of Biological Chemistry·J Noël, B Turcotte

❮ Previous
Next ❯

Citations

Aug 3, 2007·Annual Review of Genetics·Dorothy Beckett
May 5, 2011·PLoS Biology·Shulin JuGregory A Petsko
Sep 11, 2013·Genetics·Timothy R Hughes, Carl G de Boer
Aug 7, 2010·Metabolic Engineering·Brigitte GasserDiethard Mattanovich
Apr 30, 2008·Biochimica Et Biophysica Acta·Nicole R PendiniSteven W Polyak
Feb 7, 2012·Biochimica Et Biophysica Acta·Caroline C PhilpottAvery G Frey
Jan 24, 2020·Yeast·Thomas PerliJean-Marc Daran
Aug 13, 2020·Metabolites·Hongting TangJay D Keasling

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.