Viability and electrophysiology of neural cell structures generated by the inkjet printing method

Biomaterials
Tao XuThomas Boland

Abstract

Complex cellular patterns and structures were created by automated and direct inkjet printing of primary embryonic hippocampal and cortical neurons. Immunostaining analysis and whole-cell patch-clamp recordings showed that embryonic hippocampal and cortical neurons maintained basic cellular properties and functions, including normal, healthy neuronal phenotypes and electrophysiological characteristics, after being printed through thermal inkjet nozzles. In addition, in this study a new method was developed to create 3D cellular structures: sheets of neural cells were layered on each other (layer-by-layer process) by alternate inkjet printing of NT2 cells and fibrin gels. These results and findings, taken together, show that inkjet printing is rapidly evolving into a digital fabrication method to build functional neural structures that may eventually find applications in neural tissue engineering.

Citations

Jul 25, 2009·Analytical and Bioanalytical Chemistry·Young S SongUtkan Demirci
Apr 12, 2013·Chemical Society Reviews·Savas TasogluUtkan Demirci
Sep 3, 2010·Biofabrication·Karoly JakabGabor Forgacs
Mar 8, 2012·Tissue Engineering. Part a·Xiaofeng CuiDarryl D D'Lima
Jul 10, 2009·Tissue Engineering. Part C, Methods·SangJun MoonUtkan Demirci
May 18, 2012·Artificial Organs·Matthew E PepperKaren J L Burg
Apr 17, 2010·Nanomedicine·Hikmet GeckilUtkan Demirci
Mar 5, 2011·Journal of Visualized Experiments : JoVE·J Lowry CurleyMichael J Moore
Jul 24, 2012·Advanced Drug Delivery Reviews·Boris Michael HolzapfelDietmar Werner Hutmacher
Mar 26, 2013·Applied Microbiology and Biotechnology·Cameron J FerrisMarc In het Panhuis
Sep 18, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marcela KrálováJaroslav Cihlář
Oct 27, 2015·Chemical Reviews·Tomasz JungstJürgen Groll
Dec 25, 2012·Trends in Biotechnology·Savas Tasoglu, Utkan Demirci
Apr 20, 2010·Journal of Virological Methods·Lisa A FitzgeraldJames L Van Etten
Aug 22, 2009·Biomaterials·Xiaofeng Cui, Thomas Boland
Sep 25, 2009·Colloids and Surfaces. B, Biointerfaces·Mohidus Samad KhanGil Garnier
Nov 13, 2015·Biomaterials·Ibrahim T Ozbolat, Monika Hospodiuk
Aug 16, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Changhong ZhangThomas Boland
Apr 15, 2008·Biomaterials·Ke ChengWilliam S Kisaalita
Nov 21, 2007·Journal of Bioscience and Bioengineering·Akira ItoMasamichi Kamihira
Apr 7, 2009·Journal of Tissue Engineering and Regenerative Medicine·Cheryl A ParzelKaren J L Burg
Jun 10, 2009·Biotechnology Journal·Kalyani NairWei Sun
Oct 25, 2011·Biotechnology Journal·Jorge I Rodríguez-DévoraTao Xu
Oct 26, 2012·Biotechnology and Bioengineering·Guoyou HuangFeng Xu
Jan 4, 2012·Journal of Tissue Engineering and Regenerative Medicine·Sanna TurunenMinna Kellomäki
Jul 1, 2010·Biotechnology and Bioengineering·Xiaofeng CuiThomas Boland
Sep 22, 2011·Journal of Biomedical Materials Research. Part a·J Lowry Curley, Michael J Moore
Mar 23, 2013·Journal of Biomedical Materials Research. Part a·Catherine A GoubkoXudong Cao
Jan 17, 2009·Journal of Biomedical Materials Research. Part a·Hironori Yamazoe, Toshizumi Tanabe
Jun 18, 2011·Brain Research·Xianghong ArakakiMichael G Harrington
Feb 17, 2015·International Journal of Pharmaceutics·Petri IhalainenNiklas Sandler
Jun 8, 2014·Biomaterials·Anwarul HasanAli Khademhosseini
Aug 8, 2015·Stem Cells International·Catherine Baugé, Karim Boumédiene
Aug 7, 2013·Journal of the Mechanical Behavior of Biomedical Materials·Chad D MarkertMartin Guthold
Jun 20, 2016·Biomaterials·Hemanth GudapatiIbrahim Ozbolat
Jul 26, 2014·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Young-Joon SeolJames J Yoo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.