Viability, Enzymatic and Protein Profiles of Pseudomonas aeruginosa Biofilm and Planktonic Cells after Monomeric/Gemini Surfactant Treatment

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Anna KozirógBogumił Brycki

Abstract

This study set out to investigate the biological activity of monomeric surfactants dodecyltrimethylammonium bromide (DTAB) and the next generation gemini surfactant hexamethylene-1,6-bis-(N,N-dimethyl-N-dodecylammonium bromide) (C6) against the environmental strain Pseudomonas aeruginosa PB_1. Minimal inhibitory concentrations (MIC) were determined using the dilution method. The viability of the planktonic cells and biofilm was assessed using the plate count method. Enzymatic profile was determined using the API-ZYM system. Proteins were extracted from the biofilm and planktonic cells and analysed using SDS-PAGE. The MIC of the gemini surfactants was 70 times lower than that of its monomeric analogue. After 4 h of treatment at MIC (0.0145 mM for C6 and 1.013 mM for DTAB), the number of viable planktonic cells was reduce by less than 3 logarithm units. At the concentration ≥MIC, a reduction in the number of viable cells was observed in mature biofilms (p < 0.05). Treatment for 4 h with gemini surfactant at 20 MIC caused complete biofilm eradication. At sub-MIC, the concentration of some enzymes reduced and their protein profiles changed. The results of this study show that due to its superior antibacterial activity, gemini compo...Continue Reading

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Citations

Jun 14, 2020·Pathogens·Dobrawa KwaśniewskaDaria Wieczorek
Mar 22, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Silvia Bittner FialováMiloš Lukáč
Dec 6, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Martin PisárčikBranislav Horváth
Nov 4, 2021·Canadian Journal of Microbiology·Soheir A A HagrasHala A Farrag

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Datasets Mentioned

BETA
MF777034

Methods Mentioned

BETA
electrophoresis

Software Mentioned

OriginPro
SigmaGel

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