PMID: 9637827Jun 25, 1998Paper

Viability of eggs, filariform larvae and adults of Strongyloides venezuelensis (Nematoda: Strongyloidea) maintained in vitro

The Korean Journal of Parasitology
B K BaekK Matsuda

Abstract

The present study was performed to check the viability of eggs, filariform larvae and adults of Strongyloides venezuelensis exposed to various conditions for an in vitro maintenance. The eggs in the feces remained viable for about 25 days at 4 degrees C and 15 days at room temperature. However, the isolated eggs in sterile saline lost their viability within 24 hr at 4 degrees C. The eggs in morula stage were very sensitive to air drying and rapidly lost their viability (< or = 12 hr). Filariform larvae survived for a maximum period of 45 days in fecal suspension and 28 days in 0.12% nutrient broth in polyvinyl culture bags maintained at 20 degrees C. On the other hand, those isolated from nutrient broth cultures survived for a maximum period of 32 days in tap water and 22 days in sterile saline at 20 degrees C. The mature adult worms obtained from experimentally infected rats survived maximally for 9 days in serum supplemented (10% rat-serum) 0.12% nutrient broth and 4 days in serum free nutrient broth at 37 degrees C while the culture media were changed at an alternate day. The adult female worms deposited fertile eggs in serum supplemented and serum free nutrient broth cultures, however, the hatched larvae (L1) were not able ...Continue Reading

Citations

Mar 9, 2010·Experimental Parasitology·Ana Lúcia R GonçalvesJulia M Costa-Cruz
Oct 9, 2003·The Journal of Veterinary Medical Science·Kiku MatsudaByeong-Kirl Baek
Oct 22, 2011·Diagnostic Microbiology and Infectious Disease·Ana Lúcia Ribeiro GonçalvesJulia Maria Costa-Cruz

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