Viability tests of cryopreserved endocrine pancreatic cells

Cryobiology
A Andersson, S Sandler

Abstract

Collagenase-isolated islets, which had been cultured for 1 week, were frozen at two different cooling rates. Islets frozen at 5 degrees C/min behaved from a functional point of view very similarly to that of nonfrozen, cultured control islets, except for a reduced maximal insulin secretory capacity and a reduced insulin content. Slowly frozen islets (0.5 degrees C/min), however, displayed reduced rates of both proinsulin biosynthesis and glucose oxidation. It is concluded that isolated islets can be cryopreserved with great success and that the methods of choice for viability tests are those characterizing the dynamics of insulin secretory capacity of the cryopreserved islets.

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Citations

Aug 28, 2001·Journal of Biomedical Materials Research·A KingA Andersson
Jul 1, 1984·Diabetologia·A AnderssonA Schnell
Jan 1, 1993·Transplant International : Official Journal of the European Society for Organ Transplantation·J ForemanM J Taylor
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Jul 23, 1999·Annals of the New York Academy of Sciences·R V Rajotte
May 1, 1994·The Journal of Clinical Investigation·D L EizirikC Hellerström
Feb 22, 2001·Biochemical and Biophysical Research Communications·A K AnderssonS Sandler
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Jan 10, 2018·Islets·Greg G KojayanJonathan R T Lakey
Feb 1, 1992·The American Journal of Physiology·A SjöholmC Hellerström
Nov 1, 1990·The American Journal of Physiology·A SjöholmC Hellerström
Jul 31, 1995·Biochemical Pharmacology·D L Eizirik, G E Kisby

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