Viable bacteria associated with red blood cells and plasma in freshly drawn blood donations

PloS One
Christian DamgaardClaus Henrik Nielsen

Abstract

Infection remains a leading cause of post-transfusion mortality and morbidity. Bacterial contamination is, however, detected in less than 0.1% of blood units tested. The aim of the study was to identify viable bacteria in standard blood-pack units, with particular focus on bacteria from the oral cavity, and to determine the distribution of bacteria revealed in plasma and in the red blood cell (RBC)-fraction. Cross-sectional study. Blood were separated into plasma and RBC-suspensions, which were incubated anaerobically or aerobically for 7 days on trypticase soy blood agar (TSA) or blue lactose plates. For identification colony PCR was performed using primers targeting 16S rDNA. Blood donors attending Capital Region Blood Bank, Copenhagen University Hospital, Rigshospitalet, Hvidovre, Denmark, October 29th to December 10th 2013. 60 donors (≥50 years old), self-reported medically healthy. Bacterial growth was observed on plates inoculated with plasma or RBCs from 62% of the blood donations. Growth was evident in 21 (35%) of 60 RBC-fractions and in 32 (53%) of 60 plasma-fractions versus 8 of 60 negative controls (p = 0.005 and p = 2.6x10-6, respectively). Propionibacterium acnes was found in 23% of the donations, and Staphylococcu...Continue Reading

References

Sep 1, 1992·Immunopharmacology·M Pascual, J A Schifferli
Oct 1, 1995·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·H SeifertF Perdreau-Remington
Sep 12, 2001·Transfusion·S KunishimaK Ozawa
Jun 7, 2002·Archives of Surgery·Patrick J OffnerChristopher C Silliman
May 26, 2004·Immunology Letters·Helena Tlaskalová-HogenováAlena Kokesová
May 9, 2006·Journal of Clinical Periodontology·Lone FornerPalle Holmstrup
Oct 17, 2006·Clinical Microbiology Reviews·Keri K Hall, Jason A Lyman
Jul 9, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·Gabriele Walther-Wenke
Jul 16, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·Thomas Montag
Aug 6, 2008·Critical Care Medicine·Paul E Marik, Howard L Corwin
Dec 17, 2008·Journal of Medical Microbiology·Elizabeth McLellan, David Partridge
Jan 13, 2009·Clinical Microbiology Reviews·N B ParahitiyawaL P Samaranayake
Jul 18, 2009·Nature Reviews. Microbiology·Michael Otto
Aug 4, 2009·BMC Medicine·Mary A M RogersBrahmajee K Nallamothu
Sep 15, 2009·Travel Medicine and Infectious Disease·Nora G RegaladoSuresh J Antony
May 14, 2010·The New England Journal of Medicine·Anton Y Peleg, David C Hooper
Nov 19, 2011·Journal of Clinical Periodontology·Inmaculada TomásNikolaos Donos
Nov 26, 2011·Expert Review of Anti-infective Therapy·Alexandra Perry, Peter Lambert
Mar 24, 2012·Journal of Medical Microbiology·James S CargillJonathan A T Sandoe
Feb 19, 2013·Circulation·Tanja PessiJussi Mikkelsson
Mar 7, 2013·Transfusion·Mark E BrecherUNKNOWN AABB Bacterial Contamination Task Force
Apr 12, 2013·Periodontology 2000·P Mark Bartold, Thomas E Van Dyke
Sep 17, 2013·British Journal of Haematology·Paula H B Bolton-Maggs, Hannah Cohen
Jan 15, 2014·The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien Des Maladies Infectieuses Et De La Microbiologie Médicale·Wenwan ZhouJacques Genest
Mar 19, 2014·Transfusion Medicine Reviews·Richard J BenjaminUNKNOWN ISBT Transfusion Transmitted Infectious Disease Bacterial Workgroup
Apr 3, 2014·JAMA : the Journal of the American Medical Association·Jeffrey M RohdeMary A M Rogers
May 30, 2014·PloS One·Anna Carolina Ratto Tempestini HorlianaGiuseppe Alexandre Romito
Sep 23, 2014·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Sofie Strand KlausenHåkon Reikvam

❮ Previous
Next ❯

Citations

Apr 22, 2017·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Emily J BootherClaire L Shovlin
May 13, 2017·Transfusion·Pierre LauOlivier Preynat-Seauve
Jul 10, 2018·Expert Review of Molecular Diagnostics·Jean Pierre RutangaStijn Deborggraeve
Nov 17, 2017·Thorax·Claire L ShovlinUNKNOWN British Thoracic Society
Jan 24, 2019·Gut·Maria Gloria Dominguez-BelloMartin J Blaser
Aug 30, 2020·Hospital Pediatrics·Sonya C Tang GirdwoodCatherine S Forster
Jul 20, 2016·Vox Sanguinis·M TahaS Ramirez-Arcos
Sep 20, 2016·International Forum of Allergy & Rhinology·Ravi JainRichard Douglas
Dec 15, 2016·Frontiers in Medicine·Douglas B Kell, Louise C Kenny
Oct 20, 2017·Interactive Cardiovascular and Thoracic Surgery·Tuomas TauriainenFausto Biancari
Feb 20, 2019·Frontiers in Cellular and Infection Microbiology·Maria J E VisserEtheresia Pretorius
May 30, 2019·Frontiers in Cellular and Infection Microbiology·Diego J CastilloMarnie Potgieter
Feb 2, 2019·Frontiers in Microbiology·Emma WhittleDaniel Paul Tonge
May 31, 2019·British Journal of Haematology·Yesim AydinokLaurence Corash
Mar 27, 2018·Biological Reviews of the Cambridge Philosophical Society·Douglas B Kell, Etheresia Pretorius
Nov 7, 2019·Gut Microbes·Shirin Moossavi, Meghan B Azad
Feb 11, 2020·Frontiers in Cellular and Infection Microbiology·Dargham B M HammadDaniel P Tonge
Oct 7, 2020·BJS Open·J H SøbyI Gögenur
Jul 16, 2020·Proceedings of the National Academy of Sciences of the United States of America·Anvay UkidveSamir Mitragotri
Dec 10, 2020·Veterinary Sciences·Elisa ScarsellaBruno Stefanon
Jan 12, 2021·Frontiers in Immunology·Jing Jing LiLai Wei
Jan 30, 2020·Microorganisms·Liliana Simões-SilvaBenedita Sampaio-Maia
Dec 1, 2019·Liver Research·Fengyuan LiWenke Feng
Aug 5, 2021·Vox Sanguinis·Sandra Ramirez-ArcosWilliam Sheffield
Aug 8, 2021·Microorganisms·Stefan PanaiotovReni Kalfin
Aug 13, 2021·Frontiers in Microbiology·Patrizia D'AquilaDina Bellizzi
Aug 31, 2021·Cancer Letters·Haiming ChenMantang Qiu

❮ Previous
Next ❯

Methods Mentioned

BETA
blood drawn
PCR

Software Mentioned

BACTHEM

Related Concepts

Related Feeds

Cellulitis

Cellulitis (erysipelas) is a recurring and debilitating bacterial infection of the skin and underlying tissue. Discover the latest research on cellulitis here.

Babesiosis

Babesiosis is caused by parasites of the genus babesia, which are transmitted in nature by the bite of an infected tick. Discover the latest research on babesiosis here.

Related Papers

International Journal of Oral and Maxillofacial Surgery
J Thomas LambrechtJ Meyer
Schweizer Monatsschrift für Zahnmedizin = Revue mensuelle suisse d'odonto-stomatologie = Rivista mensile svizzera di odontologia e stomatologia
Beatrice GlaserJ Thomas Lambrecht
Kansenshōgaku zasshi. The Journal of the Japanese Association for Infectious Diseases
A NishineK Yoshida
© 2022 Meta ULC. All rights reserved