Vibration enhances PGE2 , IL-6, and IL-8 expression in compressed hPDL cells via cyclooxygenase pathway

Journal of Periodontology
Pussadee PhusuntornsakulChidchanok Leethanakul

Abstract

Although vibration combined with orthodontic force may accelerate orthodontic tooth movement, little is known about the mechanisms that regulate the associated cellular responses. The goal of this study was to investigate whether mechanical vibration combined with compressive force regulates cyclooxygenase (COX)-2/prostaglandin E2 (PGE2 ) and interleukin (IL)-6 and IL-8 messenger RNA (mRNA) and protein expression in human periodontal ligament (hPDL) cells via the COX pathway. The primary cultured hPDL cells were exposed to mechanical vibration, compressive force or a combination of both mechanical vibration and compressive force at 24, 48, and 72 hours. The COX-2, IL-6, IL-8, receptor activator of nuclear factor kappa-Β ligand (RANKL), and osteoprotegrin (OPG) mRNA expressions were determined using quantitative real-time polymerase chain reaction (qPCR). The PGE2 , IL-6, and IL-8 protein expressions were quantified by enzyme-linked immunosorbent assay (ELISA). To demonstrate whether the expression of PGE2 , IL-6, and IL-8 was in the COX-dependent pathway, the hPDL cells were treated with indomethacin. To determine whether PGE2 stimulated the hPDL cells to express IL-6 and IL-8, exogenous PGE2 was added to the culture media. The...Continue Reading

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Citations

Nov 27, 2019·Cell Biology International·Pussadee PhusuntornsakulChidchanok Leethanakul
Nov 23, 2020·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Birgit Rath-DeschnerAndressa V B Nogueira
Aug 11, 2020·Journal of Oral Biology and Craniofacial Research·Sutiwa BenjakulChidchanok Leethanakul

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