Vibrational circular dichroism of matrix-assisted amino acid films in the mid-infrared region

Applied Spectroscopy
Peng Zhang, Prasad L Polavarapu

Abstract

Vibrational circular dichroism (VCD) spectra in the mid-infrared region of amino acid films are reported here for the first time. Amino acid films are formed from aqueous solutions with alpha-cyclodextrin (CD) serving as the matrix to facilitate the film formation. This film method eliminates the strong interfering water absorption seen in the solution study and makes it easier to measure the VCD in the 1800-1200 cm-1 region. VCD spectra for films of six amino acids, L-alanine, L-proline, L-methionine, Lhistidine, L-phenylalanine, and L-tryptophan, are obtained. For amino acids with low solubility (L-phenylalanine and L-tryptophan), VCD could not be measured in solution, so the film method is the only means of obtaining the VCD spectra for such amino acids. For amino acids with moderate solubility (L-alanine, L-proline, L-methionine, and L-histidine), VCD spectra are also obtained in the solution state and compared with their corresponding spectra in the film state. A good correlation is found between the film and solution spectra for both absorption and VCD. The VCD spectra of L-methionine, L-histidine, L-phenylalanine, and Ltryptophan are reported here in the mid-infrared region for the first time. The present study broadens ...Continue Reading

References

Feb 28, 2001·Fresenius' Journal of Analytical Chemistry·P L Polavarapu, C Zhao
Jul 9, 2004·Biophysical Journal·Ganesh Shanmugam, Prasad L Polavarapu
Aug 19, 2004·Journal of the American Chemical Society·Ganesh Shanmugam, Prasad L Polavarapu
Sep 29, 2004·Biophysical Chemistry·Ganesh Shanmugam, Prasad L Polavarapu
Oct 22, 2004·Molecular Nutrition & Food Research·Tohru TaniguchiKenji Monde
Nov 3, 2004·Carbohydrate Research·Ana G PetrovicPrasad L Polavarapu
Jun 23, 2005·Applied Spectroscopy·Ganesh Shanmugam, Prasad L Polavarapu
Jul 22, 2005·The Journal of General Virology·Ganesh ShanmugamGerald Stubbs

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Citations

Sep 22, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Timothy A Keiderling
Jul 2, 2020·Natural Product Reports·Prasad L Polavarapu, Ernesto Santoro
May 7, 2020·Biochimica Et Biophysica Acta. Proteins and Proteomics·Hisako Sato, Izuru Kawamura
Feb 17, 2010·The Journal of Physical Chemistry. a·Zhi JiIgnacio L Garzón
Nov 8, 2013·The Journal of Physical Chemistry. B·Joanna E RodeJoanna Sadlej

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