PMID: 9194530May 1, 1997Paper

Vibrational force alters mRNA expression in osteoblasts

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
R R TjandrawinataM Hughes-Fulford

Abstract

Serum-deprived mouse osteoblastic (MC3T3E1) cells were subjected to a vibrational force modeled by NASA to simulate a space shuttle launch (7.83 G rms). The mRNA levels for eight genes were investigated to determine the effect of vibrational force on mRNA expression. The mRNA levels of two growth-related protooncogenes, c-fos and c-myc, were up-regulated significantly within 30 min after vibration, whereas those of osteocalcin as well as transforming growth factor-beta1 were decreased significantly within 3 h after vibration. No changes were detected in the levels of beta-actin, histone H4, or cytoplasmic phospholipase A2 after vibration. No basal levels of cyclooxygenase-2 expression were detected. In addition, the extracellular concentrations of prostaglandin E2 (PGE2), a potent autocrine/paracrine growth factor in bone, were not significantly altered after vibration most likely due to the serum deprivation state of the osteoblasts. In comparison with the gravitational launch profile, vibrational-induced changes in gene expression were greater both in magnitude and number of genes activated. Taken together, these data suggest that the changes in mRNA expression are due to a direct mechanical effect of the vibrational force on...Continue Reading

Citations

Jun 1, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Alexandre SemovEugenia Wang
Oct 23, 2002·Journal of Applied Physiology·Kelly JohansonTimothy G Hammond
Mar 27, 2004·The Journal of Biological Chemistry·Chuen-Mao YangChou Bing Wu
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