Vibrational microspectroscopy and imaging of molecular composition and structure during human corneocyte maturation

The Journal of Investigative Dermatology
Guojin ZhangCarol R Flach

Abstract

The outermost region of the epidermis, the stratum corneum (SC), provides an essential barrier to water loss and protects against exogenous substances. The functional integrity of the SC depends on a complex maturation and exfoliation process, which is often perturbed in skin diseases. The maturation of corneocytes isolated from different depths in healthy human SC was investigated using infrared (IR) spectroscopic imaging and Raman microscopy. Both IR and Raman spectral quality of individual corneocytes was high and revealed depth-dependent variations in molecular composition. Spectral changes were identified as arising from alterations in the concentration of the major constituents of natural moisturizing factor (NMF), important in maintaining SC hydration. A significant decrease in the concentration of NMF was observed for corneocytes isolated from superficial compared to deeper SC layers (layer 3 vs. layer 11, respectively). An IR parameter that measures the relative NMF concentration in corneocytes is introduced. The potential role of vibrational imaging to evaluate corneocyte composition and molecular structure in the treatment of NMF-related diseases is discussed.

References

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Citations

Dec 13, 2006·Analytical and Bioanalytical Chemistry·Guojin ZhangRichard Mendelsohn
Jun 29, 2010·AAPS PharmSciTech·Diksha Kaushik, Bozena Michniak-Kohn
Dec 4, 2012·Clinical, Cosmetic and Investigational Dermatology·Cila Herman
Jan 16, 2007·The Journal of Investigative Dermatology·Guojin ZhangRichard Mendelsohn
Sep 11, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Guojin ZhangRichard Mendelsohn
Apr 16, 2013·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·Kaley Myer, Howard Maibach
Oct 31, 2012·International Journal of Cosmetic Science·Carol R Flach, David J Moore
Mar 19, 2013·Biochimica Et Biophysica Acta·Jessica J Li, Christopher M Yip
May 30, 2006·Biochimica Et Biophysica Acta·Richard MendelsohnDavid J Moore
Nov 18, 2006·The Journal of Investigative Dermatology·Eiichiro YagiKouichi Nakagawa
May 6, 2014·Pharmaceutical Research·Qihong ZhangCarol R Flach

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