Vibrational spectroscopic studies on fibrinogen adsorption at polystyrene/protein solution interfaces: hydrophobic side chain and secondary structure changes

The Journal of Physical Chemistry. B
Jie WangZhan Chen

Abstract

Structural changes of fibrinogen after adsorption to polystyrene (PS) were examined at the PS/protein solution interface in situ using sum frequency generation (SFG) vibrational spectroscopy and attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR). Different behaviors of hydrophobic side chains and secondary structures of adsorbed fibrinogen molecules have been observed. Our results indicate that upon adsorption, the hydrophobic PS surface induces fast structural changes of fibrinogen molecules by aligning some hydrophobic side chains in fibrinogen so that they face to the surface. Such structural changes of fibrinogen hydrophobic side chains are local changes and do not immediately induce significant changes of the protein secondary structures. Our research also shows that the interactions between adsorbed fibrinogen and the PS surface can induce significant changes of protein secondary structures or global conformations which occur on a much longer time scale.

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Citations

Nov 16, 2007·Journal of the American Chemical Society·Xin ChenPaul S Cremer
May 28, 2010·The Journal of Physical Chemistry. B·Khoi Tan NguyenZhan Chen
Oct 31, 2009·The Journal of Physical Chemistry. B·Tobias WeidnerDavid G Castner
Sep 23, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Andrew P BoughtonZhan Chen
Feb 17, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Arthur A McClellandZhan Chen
Dec 17, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Yuwei LiuZhan Chen
Apr 7, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Albert M Hung, Samuel I Stupp
Jul 16, 2010·Proceedings of the National Academy of Sciences of the United States of America·Tobias WeidnerDavid G Castner
Jul 28, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Sergio MauriHeike Arnolds
Jul 21, 2011·Journal of Tissue Engineering and Regenerative Medicine·John D KisidayDavid D Frisbie
Mar 10, 2006·Biochimica Et Biophysica Acta·Xiaoyun Chen, Zhan Chen
Jun 24, 2010·Physical Chemistry Chemical Physics : PCCP·Li Buay KohSubbu S Venkatraman
Aug 18, 2010·Physical Chemistry Chemical Physics : PCCP·Kailash C Jena, Dennis K Hore
Sep 4, 2008·Biomedical Materials·M YaseenJ R Lu
Jun 4, 2013·Physical Chemistry Chemical Physics : PCCP·Tobias Weidner, David G Castner
May 8, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Mark A EvenZhan Chen
Aug 1, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Shuji YeZhan Chen
Jul 12, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Pranav SomanChristopher A Siedlecki
Jun 8, 2021·Frontiers in Microbiology·Radha P SomarathneNicholas C Fitzkee
Jul 14, 2021·The Journal of Physical Chemistry. B·Wen GuoZhan Chen
Apr 2, 2011·Journal of the American Chemical Society·George J HolingaGabor A Somorjai
Jun 12, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Ting LinZhan Chen
Apr 26, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Kathrin EngelhardtWolfgang Peukert

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