Vibrator and PI4KIIIα govern neuroblast polarity by anchoring non-muscle myosin II

ELife
Chwee Tat KoeHongyan Wang

Abstract

A central feature of most stem cells is the ability to self-renew and undergo differentiation via asymmetric division. However, during asymmetric division the role of phosphatidylinositol (PI) lipids and their regulators is not well established. Here, we show that the sole type I PI transfer protein, Vibrator, controls asymmetric division of Drosophilaneural stem cells (NSCs) by physically anchoring myosin II regulatory light chain, Sqh, to the NSC cortex. Depletion of vib or disruption of its lipid binding and transfer activities disrupts NSC polarity. We propose that Vib stimulates PI4KIIIα to promote synthesis of a plasma membrane pool of phosphatidylinositol 4-phosphate [PI(4)P] that, in turn, binds and anchors myosin to the NSC cortex. Remarkably, Sqh also binds to PI(4)P in vitro and both Vib and Sqh mediate plasma membrane localization of PI(4)P in NSCs. Thus, reciprocal regulation between Myosin and PI(4)P likely governs asymmetric division of NSCs.

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Citations

Jun 7, 2019·PLoS Biology·Phuong Thao LyHongyan Wang
Mar 19, 2020·Frontiers in Cell and Developmental Biology·Marie ClémotD Leanne Jones
Jun 12, 2020·Mechanisms of Ageing and Development·Rafael Sênos DemarcoD Leanne Jones
Jun 11, 2021·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Tim G AshlinShamshad Cockcroft
Aug 8, 2021·International Journal of Molecular Sciences·Nadezhda BarvitenkoAntonella Pantaleo

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Methods Mentioned

BETA
transgenic
Fluorescence
fluorescence complementation
proximity ligation assay
PCR
affinity purification
transfection
bimolecular fluorescence complementation assay
confocal microscopy

Software Mentioned

EMBOSS Needle
ImageJ

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