Vibrio aestuarianus zinc metalloprotease causes lethality in the Pacific oyster Crassostrea gigas and impairs the host cellular immune defenses

Fish & Shellfish Immunology
Yannick LabreucheJean-Louis Nicolas

Abstract

Extracellular products (ECPs) of the pathogenic Vibrio aestuarianus 01/32 were previously reported to display lethality in Crassostrea gigas oysters and to cause morphological changes and immunosuppression in oyster hemocytes. To identify the source of this toxicity, biochemical and genetic approaches were developed. ECP protease activity and lethality were shown to be significantly reduced following incubation with metal chelators, suggesting the involvement of a zinc metalloprotease. An open reading frame of 1836 bp encoding a 611-aa metalloprotease (designated Vam) was identified. The deduced protein sequence showed high homology to other Vibrio metalloproteases reported to be involved in pathogenicity. To further confirm the role of this enzyme in ECP toxicity, a plasmid carrying the vam gene under the control of an araC-P(BAD) expression cassette was transferred to a Vibrio splendidus related strain, LMG20012(T), previously characterized as non-pathogenic to oysters. Expression of Vam conferred a toxic phenotype to LMG20012(T) ECPs in vivo and cytotoxicity to oyster hemocytes in vitro. Collectively, these data suggest that the Vam metalloprotease is a major contributor to the toxicity induced by V. aestuarianus ECPs and is...Continue Reading

Citations

Jan 12, 2013·Microbial Ecology·Crystal N Johnson
Apr 7, 2011·Biocontrol Science·Sumio Shinoda, Shin-Ichi Miyoshi
Nov 12, 2014·Environmental Microbiology·D GoudenègeF Le Roux
Jan 1, 2016·Developmental and Comparative Immunology·Yosra Ben CheikhFrank Le Foll
Jun 13, 2014·Environmental Microbiology·Audrey Sophie VanhoveDelphine Destoumieux-Garzón
Apr 4, 2016·Trends in Microbiology·Frédérique Le RouxMartin F Polz
Jul 27, 2015·Journal of Invertebrate Pathology·Valérie Barbosa SolomieuMarie-Agnès Travers
Jul 27, 2015·Journal of Invertebrate Pathology·Marie-Agnès TraversCarolyn S Friedman
Apr 15, 2014·Environmental Microbiology·Luigi VezzulliCarla Pruzzo
Dec 10, 2014·The ISME Journal·Astrid LemireFrédérique Le Roux
Nov 18, 2014·PloS One·Stéphanie MadecJean-Louis Nicolas
Sep 8, 2004·Journal of Virological Methods·T RenaultC Lipart
Nov 3, 2006·Journal of Virological Methods·Frederico M BatistaTristan Renault
Sep 13, 2017·The ISME Journal·Anny CárdenasChristian R Voolstra
Jul 6, 2018·Environmental Microbiology·Leila ParizadehMarie-Agnès Travers
Jun 22, 2019·Proceedings of the National Academy of Sciences of the United States of America·Tristan RubioDelphine Destoumieux-Garzón
May 5, 2020·Environmental Microbiology·Delphine Destoumieux-GarzónLuigi Vezzulli
Sep 27, 2019·Frontiers in Microbiology·K Mathias WegnerFrédérique Le Roux
May 19, 2017·Frontiers in Microbiology·Javier DubertJesús L Romalde
Aug 26, 2018·Probiotics and Antimicrobial Proteins·B HouyvetC Zatylny-Gaudin
Jun 20, 2019·Frontiers in Cellular and Infection Microbiology·Han-Jie GuLi Sun
Jul 28, 2019·Journal of Invertebrate Pathology·Qinggang Xue

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.