Apr 1, 2020

Vibrio campbellii DS40M4 is a tractable model strain that diverges from the canonical quorum-sensing regulatory circuit in vibrios

BioRxiv : the Preprint Server for Biology
C. A. SimpsonJulia C van Kessel

Abstract

Vibrio campbellii BB120 (previously designated as Vibrio harveyi) is a fundamental model strain for studying population density-based cell-to-cell communication, known as quorum sensing. In V. campbellii BB120, sensing of autoinducers at high cell densities activates the expression of the master transcriptional regulator, LuxR, which controls the expression of genes involved in group behaviors. The environmental isolate Vibrio campbellii DS40M4 was recently shown to be capable of natural transformation, a process by which bacteria take up exogenous DNA and incorporate it into their genome via homologous recombination. In contrast, BB120 is not naturally transformable. Here, we compare additional phenotypes between these two V. campbellii strains. DS40M4 has a faster growth rate and stronger type VI secretion-mediated cell killing, whereas BB120 forms more robust biofilms and is bioluminescent. To explore the function of DS40M4-encoded homologs of the BB120 quorum-sensing system, we exploited the power of natural transformation to rapidly generate >30 mutant strains. Our results show that DS40M4 has a similar quorum-sensing circuit to BB120 but with three distinct differences: 1) DS40M4 lacks the canonical HAI-1 autoinducer LuxM...Continue Reading

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Mentioned in this Paper

Exertion
Cessation of Life
NADH
Adverse Effects
Pharmaceutical Preparations
Clinical Trials
Asthenia
Pathology
Malignant Neoplasms
Metabolite

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