Vibrio cholerae non-O1, non-O139 strains isolated before 1992 from Varanasi, India are multiple drug resistant, contain intSXT, dfr18 and aadA5 genes

Environmental Microbiology
Harapriya MohapatraDurg V Singh

Abstract

In this study, we report the presence of the SXT element and Class I integron in Vibrio cholerae non-O1, non-O139 strains isolated from Varanasi, India. Isolates were resistant to cotrimoxazole, trimethoprim and/or streptomycin, furazolidone and ampicillin. None contained plasmids. Polymerase chain reaction (PCR) and DNA sequencing revealed the presence of antibiotic resistance gene cassettes, aadA1, aadA2, aadA5 and dfrA15, in the Class I integron and SXT, an integrative conjugative element containing dfr18, sulII and strAB, in three and six of the isolates respectively. Conjugation experiments, followed by PCR analysis of transconjugants, provided evidence for the transferable nature of intSXT and associated antibiotic resistance gene cassettes. This is the first report of the occurrence of SXT ICE, dfr18, sulII, strAB and aadA5 genes in environmental V. cholerae non-O1, non-O139 strains from Varanasi, India, that had been isolated before 1992.

References

Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Dec 1, 1995·Microbiology·G D Recchia, R M Hall
Apr 27, 1999·Molecular Microbiology·B Hochhut, M K Waldor
Oct 16, 2001·Antimicrobial Agents and Chemotherapy·B HochhutM K Waldor
Nov 2, 2002·Molecular Microbiology·Vincent BurrusGérard Guédon
Apr 3, 2004·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A C Fluit, F-J Schmitz
Jun 25, 2004·Antimicrobial Agents and Chemotherapy·Masaaki IwanagaMasahiko Ehara
Apr 7, 2006·Applied and Environmental Microbiology·Vincent BurrusMatthew K Waldor
Sep 19, 2006·The Journal of Antimicrobial Chemotherapy·Daniela CeccarelliMauro Maria Colombo
Jun 1, 1974·The Journal of Hygiene·E S Anderson, E J Threlfall

❮ Previous
Next ❯

Citations

Jan 9, 2009·Applied Microbiology and Biotechnology·Xu-Xiang ZhangHerbert H P Fang
Aug 15, 2013·Journal of Biotechnology·Dong YangJun-Wen Li
Nov 13, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J SunY Ni
Apr 15, 2018·Applied and Environmental Microbiology·Audrey BioteauVincent Burrus
May 1, 2009·Molecular Ecology Resources·John P Jakupciak, Rita R Colwell
Jan 23, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Murari BhandariFlavia Huygens
Aug 20, 2021·Current Microbiology·Rajeev Ranjan, Shashidhar Thatikonda

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.