Vibrio cholerae phosphoenolpyruvate phosphotransferase system control of carbohydrate transport, biofilm formation, and colonization of the germfree mouse intestine.

Infection and Immunity
Laetitia HouotPaula I Watnick

Abstract

The bacterial phosphoenolpyruvate phosphotransferase system (PTS) is a highly conserved phosphotransfer cascade whose components modulate many cellular functions in response to carbohydrate availability. Here, we further elucidate PTS control of Vibrio cholerae carbohydrate transport and activation of biofilm formation on abiotic surfaces. We then define the role of the PTS in V. cholerae colonization of the adult germfree mouse intestine. We report that V. cholerae colonizes both the small and large intestines of the mouse in a distribution that does not change over the course of a month-long experiment. Because V. cholerae possesses many PTS-independent carbohydrate transporters, the PTS is not essential for bacterial growth in vitro. However, we find that the PTS is essential for colonization of the germfree adult mouse intestine and that this requirement is independent of PTS regulation of biofilm formation. Therefore, competition for PTS substrates may be a dominant force in the success of V. cholerae as an intestinal pathogen. Because the PTS plays a role in colonization of environmental surfaces and the mammalian intestine, we propose that it may be essential to successful transit of V. cholerae through its life cycle of...Continue Reading

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Citations

Nov 23, 2011·Proceedings of the National Academy of Sciences of the United States of America·Alexandra E Purdy, Paula I Watnick
Jun 4, 2013·Applied and Environmental Microbiology·Patrick Ymele-LekiPaula I Watnick
Jun 27, 2013·Infection and Immunity·Jessica Queen, Karla J F Satchell
Feb 14, 2012·Journal of Bacteriology·Jorge Frias-Lopez, Ana Duran-Pinedo
Sep 2, 2010·PloS One·Valeriy PoroykoMichael J Morowitz
Sep 9, 2011·PLoS Pathogens·Cedric AbsalonPaula I Watnick
Mar 8, 2016·Nature Reviews. Microbiology·Andrew J Olive, Christopher M Sassetti
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Jan 8, 2018·Microbial Genomics·Inès LevadeB Jesse Shapiro
Jul 25, 2018·Journal of Bacteriology·Kambiz Morabbi Heravi, Josef Altenbuchner
Sep 19, 2018·Journal of Bacteriology·Itai MuzhingiAlexandra E Purdy
Oct 28, 2017·Microbiology·Tanner ByerJane M Liu
Nov 27, 2019·Nature Communications·Kyoo HeoYeong-Jae Seok

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