Vibrio fischeri Biofilm Formation Prevented by a Trio of Regulators

Applied and Environmental Microbiology
Cecilia M ThompsonKaren L Visick

Abstract

Biofilms, complex communities of microorganisms surrounded by a self-produced matrix, facilitate attachment and provide protection to bacteria. A natural model used to study biofilm formation is the symbiosis between Vibrio fischeri and its host, the Hawaiian bobtail squid, Euprymna scolopes Host-relevant biofilm formation is a tightly regulated process and is observed in vitro only with strains that have been genetically manipulated to overexpress or disrupt specific regulators, primarily two-component signaling (TCS) regulators. These regulators control biofilm formation by dictating the production of the symbiosis polysaccharide (Syp-PS), the major component of the biofilm matrix. Control occurs both at and below the level of transcription of the syp genes, which are responsible for Syp-PS production. Here, we probed the roles of the two known negative regulators of biofilm formation, BinK and SypE, by generating double mutants. We also mapped and evaluated a point mutation using natural transformation and linkage analysis. We examined traditional biofilm formation phenotypes and established a new assay for evaluating the start of biofilm formation in the form of microscopic aggregates in shaking liquid cultures, in the abse...Continue Reading

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Citations

Oct 10, 2018·Molecular Microbiology·Cecilia M ThompsonKaren L Visick
Oct 9, 2019·Current Opinion in Microbiology·Clotilde Bongrand, Edward G Ruby
Jan 6, 2021·Applied and Environmental Microbiology·Joshua J CohenKaren L Visick
Jun 6, 2021·Nature Reviews. Microbiology·Karen L VisickEdward G Ruby

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