Vibrio parahaemolyticus orchestrates a multifaceted host cell infection by induction of autophagy, cell rounding, and then cell lysis.

Proceedings of the National Academy of Sciences of the United States of America
Dara L BurdetteKim Orth

Abstract

The bacterial pathogen Vibrio parahaemolyticus utilizes a type III secretion system to cause death of host cells within hours of infection. We report that cell death is completely independent of apoptosis and occurs by a mechanism in which injection of multiple type III effectors causes induction of autophagy, cell rounding, and the subsequent release of cellular contents. Autophagy is detected by the appearance of lipidated light chain 3 (LC3) and by increases in punctae and vacuole formation. Electron microscopy reveals the production of early autophagic vesicles during infection. Consistent with phosphoinositide 3 (PI3) kinase playing a role in autophagy, treatment of infected cells with a PI3 kinase inhibitor attenuates autophagy in infected cells. Because many effectors are injected during a V. parahaemolyticus infection, it is not surprising that the presence of a sole PI3 kinase inhibitor does not prevent inevitable host-cell death. Our studies reveal an infection paradigm whereby an extracellular pathogen uses its type III secretion system to cause at least three parallel events that eventually result in the proinflammatory death of an infected host cell.

References

Feb 7, 1985·The New England Journal of Medicine·J G Morris, R E Black
Sep 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·D M MonackS Falkow
May 24, 2000·The Journal of Infectious Diseases·N A DanielsL Slutsker
Oct 13, 2000·Molecular Microbiology·M A Brennan, B T Cookson
Mar 20, 2004·Nature Reviews. Microbiology·Karla KirkegaardWilliam T Jackson
Jun 1, 2004·Journal of Cell Science·Yukiko KabeyaTamotsu Yoshimori
Aug 25, 2004·The International Journal of Biochemistry & Cell Biology·Isei TanidaEiki Kominami
Oct 27, 2004·Infection and Immunity·Kwon-Sam ParkTakeshi Honda
Dec 4, 2004·Science·Michinaga OgawaChihiro Sasakawa
Dec 14, 2004·Microbiology and Molecular Biology Reviews : MMBR·Partho Ghosh
Feb 8, 2005·Current Opinion in Microbiology·Lorena NavarroJack E Dixon
Jun 10, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yue ZhangJames B Bliska
Oct 4, 2005·The Journal of Clinical Investigation·Beth Levine, Junying Yuan
Jan 24, 2006·Infection and Immunity·Takahiro OnoTakeshi Honda
Feb 3, 2007·Proceedings of the National Academy of Sciences of the United States of America·Maximiliano Gabriel GutierrezMaría Isabel Colombo
Apr 7, 2007·Trends in Biochemical Sciences·Sohini MukherjeeKim Orth
Sep 4, 2007·Nature Reviews. Immunology·Beth Levine, Vojo Deretic
Sep 21, 2007·The Journal of Biological Chemistry·Jennifer E TroskyKim Orth
Oct 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Amy D B LivermanKim Orth
Nov 9, 2007·Apoptosis : an International Journal on Programmed Cell Death·Andrew Thorburn
Jan 15, 2008·Cell·Beth Levine, Guido Kroemer
Jan 16, 2008·PLoS Pathogens·Edison MejíaGloria I Viboud

❮ Previous
Next ❯

Citations

Jan 12, 2013·Microbial Ecology·Crystal N Johnson
Nov 1, 2011·Apoptosis : an International Journal on Programmed Cell Death·Pei-Yin YangJune Hsieh Wu
Sep 6, 2008·Cell Death and Differentiation·A Orvedahl, B Levine
Jun 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Anne Marie KrachlerKim Orth
Jun 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Anju SreelathaVincent J Starai
Apr 23, 2010·The Journal of Biological Chemistry·Phi LuongKim Orth
Dec 24, 2010·The Journal of Infectious Diseases·Takaaki ShimohataAkira Takahashi
Oct 14, 2008·Applied and Environmental Microbiology·Michelle A Laskowski-Arce, Kim Orth
Mar 28, 2012·Annual Review of Immunology·Petric KuballaRamnik J Xavier
Jan 17, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Wanida MalaChariya Chomvarin
Feb 26, 2013·Current Opinion in Microbiology·Lingling Zhang, Kim Orth
Jan 8, 2013·International Journal of Food Microbiology·Li WangDongsheng Zhou
Jul 25, 2009·Molecular Microbiology·Dara L BurdetteKim Orth
Apr 20, 2011·Cellular Microbiology·Vanessa Liévin-Le MoalAlain L Servin
Aug 29, 2013·International Journal of Medical Microbiology : IJMM·Nicky O'BoyleAoife Boyd
Jul 26, 2011·Microbes and Infection·Christopher A BrobergKim Orth
Mar 24, 2015·Frontiers in Microbiology·Rongzhi WangShihua Wang
Apr 9, 2015·Frontiers in Microbiology·Tracy H HazenPatricia A Sobecky
Jun 17, 2009·Cell Host & Microbe·Vojo Deretic, Beth Levine
Jan 8, 2015·Frontiers in Cellular and Infection Microbiology·Shimaa S El-MalahXinan Jiao
May 6, 2016·Annual Review of Cell and Developmental Biology·Alyssa JimenezNeal M Alto
Sep 25, 2015·Scientific Reports·Ming Liu, Sheng Chen

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis