Vicenin-2 inhibits Wnt/β-catenin signaling and induces apoptosis in HT-29 human colon cancer cell line

Drug Design, Development and Therapy
Dong YangThamaraiselvan Rengarajan

Abstract

Colorectal cancer (CRC) is among highest prevailing cancers in the whole world, especially in western countries. For a diverse of reasons, patients prefer naturally occurring dietary substances over synthetic agents to prevent cancer. Vicenin-2 is largely available in a medicinal plant Ocimum sanctum and is an apigenin form, 6,8-di-C-glucoside, which has been reported to have a range of pharmacological values which includes antioxidant, hepatoprotective, anti-inflammatory and anti-cancer. This study was aimed to analyze the anti-proliferative effect of Vicenin-2 on human colon cancer cells via the Wnt/β-catenin signaling inhibition. MTT assay was used to assess the cell viability at different concentrations and time point. Vicenin-2 at a concentration of 50 µM (IC50) decreased the phosphorylated (inactive) glycogen synthase kinase-3β, cyclin D1, and non-p-β-catenin expressions in HT-29 cells, which were evidenced through western blot analysis. Further, Vincenin-2 reduced the T-cell factor (TCF) / Leukocyte erythroid factor (LEF) reporter activity in HT-29 cells. Vicenin-2 also promoted substantial cell cycle arrest at the G2M phase of HT-29 cells, as well induced apoptosis in HT-29 cells, as revealed through flow cytometric ana...Continue Reading

Citations

Apr 12, 2019·Drug Development Research·Yuti YinWenhui Fang
Aug 28, 2019·Preparative Biochemistry & Biotechnology·Georgia de Assis Dias AlvesMaria José Vieira Fonseca
Apr 8, 2020·BioMed Research International·Shengli YuanDapeng Wu
Nov 22, 2019·Anti-cancer Agents in Medicinal Chemistry·Rumana AhmadArvind K Srivastava
Dec 15, 2020·Journal of Biochemical and Molecular Toxicology·Yijun LiHuibo Wang

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Methods Mentioned

BETA
ubiquitination
Flow
FACS
transfection
Assay
flow cytometry

Software Mentioned

Statistical Package for Students
FACS Calibur system
ImageJ
Cell Quest

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