PMID: 3214745Sep 1, 1988Paper

Videomicroscopy of acute brain slices from amygdala and hippocampus

Brain Research Bulletin
C L KeenanT H Brown

Abstract

Video-enhanced contrast, differential-interference contrast microscopy (VEC-DICM) was used to visualize the cytoarchitecture and subcellular neuronal structure of acute brain slices from rat hippocampus and amygdala. Even at low-power magnification, the VEC-DICM system vastly improved our ability to visualize and examine the gross organization of the tissue. With medium-power magnification, the neuronal somata and proximal dendrites were clearly visible. With high-power magnification, some of the subcellular details could be clearly discerned--including cell nuclei, cell nucleoli, fine dendritic processes, and varicosities that may be synaptic expansions. We conclude that improved optical techniques should be valuable to cellular neurobiologists interested in structure-function relationships in brain slices.

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Citations

May 15, 2002·Hippocampus·James R MoyerThomas H Brown
Jan 1, 1990·Synapse·P F ChapmanT H Brown
Jun 1, 1993·Pflügers Archiv : European journal of physiology·G J StuartB Sakmann
Feb 1, 1992·Developmental Biology·W R Eckberg, R E Palazzo
Dec 24, 1990·Brain Research·H U Dodt, W Zieglgänsberger
Jul 28, 1999·The Journal of Comparative Neurology·B Faulkner, T H Brown
Dec 15, 1994·Annals of the New York Academy of Sciences·T H Brown, D B Jaffe
Jul 1, 1997·Journal of Neurophysiology·D B Jaffe, T H Brown
Nov 4, 1988·Science·T H BrownC L Keenan

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