PMID: 8980957Dec 1, 1996Paper

Videomicroscopy of methacholine-induced contraction of individual airways in precision-cut lung slices

The European Respiratory Journal
C MartinV Ullrich

Abstract

Contraction of airways of different size can be studied in viable lung slices by videomicroscopy. However, at present, application of this technique is limited by the heterogeneous responses obtained. We investigated the use of precision-cut lung slices to examine contraction of individual airways. Lung slices of 250 +/- 20 microns were prepared from Wistar rats and cultured in a roller incubator in serum-free minimum essential medium (MEM). Under these conditions, the slices were viable for at least 70 h, as indicated by leakage of lactate dehydrogenase into the supernatant, thymidine incorporation and ciliary beating. The slices were placed in a newly developed incubation chamber and mounted by a nylon thread that was fixed to a platinum wire. The whole chamber was positioned on a microscope stage, and contraction of single airways was followed under a microscope that was coupled to a CCD-camera. Reduction in airway area was taken as an index of bronchoconstriction and was determined by a computer program. Addition of methacholine resulted in a concentration-dependent (concentration producing half the maximal effect (EC50) = 0.64 +/- 0.08 (mean +/- SD) microM; n = 64) contraction of single airways. In the presence of hydrocor...Continue Reading

Citations

May 25, 2011·Annals of Biomedical Engineering·S M K RauschW A Wall
Jan 26, 2007·Veterinary Research Communications·J VietmeierM Kietzmann
Feb 5, 2004·Regulatory Peptides·Jochen SpringerAxel Fischer
Aug 26, 1998·Respiration Physiology·J Van de Voorde, G Joos
Apr 16, 1999·British Journal of Pharmacology·H D HeldS Uhlig
Jan 30, 2002·The Journal of General Physiology·Albrecht Bergner, Michael J Sanderson
Dec 22, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Heike SchlenzGabriela Krasteva
Nov 25, 2011·Journal of Applied Physiology·Jian ZhouSteven C George
Nov 16, 2006·International Archives of Allergy and Immunology·Julia KellnerAlbrecht Bergner
May 24, 2001·American Journal of Respiratory and Critical Care Medicine·A WohlsenC Martin
Feb 13, 2001·American Journal of Respiratory Cell and Molecular Biology·C MartinV Ullrich
Aug 26, 2006·American Journal of Respiratory Cell and Molecular Biology·Yan BaiMichael J Sanderson
Sep 29, 2009·American Journal of Respiratory Cell and Molecular Biology·Philip R CooperR Graham Sturton
Nov 4, 2004·Respiratory Research·David A Groneberg, K Fan Chung
Mar 1, 2006·Respiratory Research·Albrecht BergnerRudolf M Huber
Sep 12, 2013·PloS One·David M L LilburnThomas Meersmann
Dec 3, 2013·Pulmonary Pharmacology & Therapeutics·M FitzPatrickJ E Bourke
Dec 10, 2013·American Journal of Respiratory Cell and Molecular Biology·Sonia R RosnerRamaswamy Krishnan
Sep 13, 2012·Pulmonary Pharmacology & Therapeutics·David WrightAdrian R West
Aug 10, 2011·Pulmonary Pharmacology & Therapeutics·Robin J McAnulty
May 24, 2011·Pulmonary Pharmacology & Therapeutics·Michael J Sanderson
Mar 15, 2011·Journal of the Mechanical Behavior of Biomedical Materials·S M K RauschW A Wall
Dec 31, 2008·Pulmonary Pharmacology & Therapeutics·Deborah L ClarkeAkira Yamasaki
Dec 2, 2008·Pulmonary Pharmacology & Therapeutics·P R CooperA R West
Jun 3, 2008·Trends in Pharmacological Sciences·Graham SturtonPeter J Barnes
Jun 2, 2007·European Journal of Pharmacology·Renato FaroJane A Mitchell
Dec 16, 2006·Pulmonary Pharmacology & Therapeutics·Carl G A Persson
Jul 15, 2006·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·V J ErpenbeckJ M Hohlfeld
Dec 4, 2012·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Katherina Sewald, Armin Braun
Feb 12, 2013·European Journal of Pharmacology·Stephanie M BrownLouise E Donnelly
Nov 8, 2016·Scientific Reports·Pinelopi AndrikakouHari Arora
Mar 15, 2012·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Su Jung ParkSung Joon Kim
Jan 22, 2015·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Hae Jin KimSung Joon Kim

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