PMID: 2120098Oct 1, 1990Paper

Viewing molecules with scanning tunneling microscopy and atomic force microscopy

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
R D EdstromD F Evans

Abstract

Two new microscopic techniques make it possible to obtain images of biologically interesting molecules directly in air, vacuum, or under water. Scanning tunneling microscopy and atomic force microscopy both have the capacity to visualize atoms on the surface of rigid structures and provide details of molecular structure for lipids, proteins, carbohydrates, and nucleic acids. In addition to providing visualizations of individual molecules, these scanning probe techniques allow direct imaging of complexes between molecules or between molecules and higher-order subcellular structures such as membranes and cytoskeletal components. Both microscopes can be operated under a variety of ambient conditions ranging from high vacuum to above atmospheric pressure. Specimens need not be dry; both techniques have been used to image molecules in aqueous media under nearly physiological conditions. It is proposed that as these techniques mature they will allow direct observation of many molecular interactions under physiological conditions or even in vivo while they are occurring within the cell.

Citations

Oct 2, 2007·The Review of Scientific Instruments·Eugenia PechkovaClaudio Nicolini
Nov 1, 1993·Chromosoma·M J AllenR Balhorn
Jan 1, 1994·Progress in Biophysics and Molecular Biology·V J Morris

❮ Previous
Next ❯

Related Concepts

Related Feeds

AFM in situ DNA

AFM in situ DNA describes in situ analysis (or study) of DNA using atomic force microscopy. Discover the latest research on AFM in situ DNA here.

Related Papers

Forensic Science International : Synergy
S KasasG Dietler
Micron : the International Research and Review Journal for Microscopy
M Firtel, T J Beveridge
Proceedings of the National Academy of Sciences of the United States of America
D P AllisonR J Warmack
© 2021 Meta ULC. All rights reserved