Viewing of complex molecules of ethidium bromide and plasmid DNA in solution by atomic force microscopy

Chemical & Pharmaceutical Bulletin
K UtsunoT Iwamoto

Abstract

Tertiary structure changes in plasmid DNA, induced by ethidium bromide intercalation, have been observed in aqueous solutions by the use of an atomic force microscope. A relaxed closed circular pBR322 molecule became a positively supercoiled complex on the drug binding. The supercoiling always resulted in an interwound (or a plectonemic) form, but never a solenoidal (or a toroidal) form. A quantitative analysis of the compactness of such supercoiled complexes has been carried out.

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Citations

Nov 13, 2004·European Biophysics Journal : EBJ·Olivier PiétrementEric Le Cam
Feb 19, 2002·Chemical & Pharmaceutical Bulletin·Kuniharu UtsunoToschitake Iwamoto
Apr 6, 2006·Chemical & Pharmaceutical Bulletin·Yangzhe WuYong Chen
Jul 19, 2011·Analytical and Bioanalytical Chemistry·Marek MahutWolfgang Lindner
Mar 20, 2010·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Naoko KidaKenichi Yoshikawa
Dec 10, 2015·European Biophysics Journal : EBJ·M KopaczynskaJ H Fuhrhop
Jul 8, 2008·Biochemical and Biophysical Research Communications·Jia YuXitai Huang
Oct 9, 2007·Advanced Drug Delivery Reviews·Ya Tsz A TurnerMartyn C Davies
Dec 14, 2004·Journal of Molecular Biology·Yolanda D TsengRobert M Henderson
Feb 5, 2003·Biochemical and Biophysical Research Communications·Miriam ArgamanEsther Priel
Oct 12, 2016·Chemical & Pharmaceutical Bulletin·Kuniharu UtsunoHasan Uludağ

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