Viewpoint: Carbon isotope effect predictions for enzymes involved in the primary carbon metabolism of plant leaves

Functional Plant Biology : FPB
Guillaume Tcherkez, G D Farquhar

Abstract

Carbon isotope effects of enzymes involved in primary carbon metabolism are key parameters in our understanding of plant metabolism. Nevertheless, some of them are poorly known because of the lack of in vitro experimental data on purified enzymes. Some studies have focused on theoretical predictions of isotope effects. Here we show how quantum chemical calculations can be adapted for calculation of isotope effects for the Rubisco-catalysed carboxylation and oxygenation reactions and the citrate synthase reaction. The intrinsic isotope effect of the carboxylation by Rubisco appears to be much smaller than previously thought, being close to the overall isotope effect of the reaction that is, between 25 and 30 per mil. The same applies to the enzyme citrate synthase, that catalyses the first step of the Krebs cycle, with an isotope effect of around 23 per mil. Combined with the isotope effects of equilibrium reactions calculated with β-factors, the Krebs cycle then has an overall isotope effect that depletes organic acids in 13C.

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Citations

Apr 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Guillaume G B TcherkezT John Andrews
Apr 9, 2014·Journal of Experimental Botany·Susanne von CaemmererAsaph B Cousins
Dec 15, 2018·Tree Physiology·Millicent R Smith, A Merchant
Jul 26, 2019·Physical Chemistry Chemical Physics : PCCP·Mark A Iron, Jonathan Gropp
Jun 1, 2006·Functional Plant Biology : FPB·Guillaume Tcherkez, Graham D Farquhar
Mar 1, 2007·Functional Plant Biology : FPB·Margaret M Barbour
Nov 22, 2008·The New Phytologist·Camille BathellierJaleh Ghashghaie
Jan 20, 2010·Plant, Cell & Environment·Guillaume TcherkezJaleh Ghashghaie
Jan 9, 2008·The New Phytologist·David R BowlingJames T Randerson
Oct 20, 2010·Plant, Cell & Environment·Guillaume TcherkezAgnes Grapin
Aug 20, 2015·Plant, Cell & Environment·Guillaume Tcherkez
Nov 21, 2013·The New Phytologist·Jaleh Ghashghaie, Franz W Badeck
Jun 25, 2015·Proceedings of the National Academy of Sciences of the United States of America·Katarzyna M RomekRichard J Robins
Jun 4, 2008·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·Béla BarabásGyula Pályi
May 1, 2008·Functional Plant Biology : FPB·Guillaume Tcherkez, Graham D Farquhar
May 27, 2010·Journal of Experimental Botany·Christopher P BickfordNate G McDowell
Aug 14, 2012·Plant, Cell & Environment·John R Evans, Susanne von Caemmerer
Apr 22, 2015·Isotopes in Environmental and Health Studies·Hanns-Ludwig SchmidtRoland A Werner
Jun 1, 2008·Functional Plant Biology : FPB·Iker AranjueloSalvador Nogués
Jan 7, 2015·Nature Chemical Biology·Paulo DurãoManajit Hayer-Hartl
Jul 16, 2009·Rapid Communications in Mass Spectrometry : RCM·Christiane WernerPierrick Priault
Oct 17, 2008·Plant Physiology·Gary J LaniganUlli Seibt
Aug 21, 2012·Journal of the American Chemical Society·Rupam SarmaJustine P Roth

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Methods Mentioned

BETA
nuclear magnetic resonance

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