Viewpoint: How large is the carbon isotope fractionation of the photorespiratory enzyme glycine decarboxylase?

Functional Plant Biology : FPB
Guillaume G B Tcherkez

Abstract

Despite the intense effort developed over the past 10 years to determine the 12C / 13C isotope fractionation associated with photorespiration, much uncertainty remains about the amplitude, and even the sign, of the 12C / 13C isotope fractionation of glycine decarboxylase, the enzyme that produces CO2 during the photorespiratory cycle. In fact, leaf gas-exchange data have repeatedly indicated that CO2 evolved by photorespiration is depleted in 13C compared with the source material, while glycine decarboxylase has mostly favoured 13C in vitro. Here I give theoretical insights on the glycine decarboxylase reaction and show that (i), both photorespiration and glycine decarboxylation must favour the same carbon isotope - the in vitro measurements being probably adulterated by the high sensitivity of the enzyme to assay conditions and the possible reversibility of the reaction in these conditions, and (ii), simplified quantum chemistry considerations as well as comparisons with other pyridoxal 5'-phosphate-dependent decarboxylases indicate that the carbon isotope fractionation favour the 12C isotope by ~20‰, a value that is consistent with the value of the photorespiratory fractionation (f) obtained by gas-exchange experiments.

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Jun 1, 2006·Functional Plant Biology : FPB·Guillaume Tcherkez, Graham D Farquhar

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Citations

Sep 5, 2020·Isotopes in Environmental and Health Studies·Shiva GhiasiRoland A Werner
May 27, 2010·Journal of Experimental Botany·Christopher P BickfordNate G McDowell
Aug 2, 2013·The New Phytologist·Lucas A CernusakGraham D Farquhar
Jun 18, 2015·Plant, Cell & Environment·Iker AranjueloGuillaume Tcherkez
May 28, 2011·American Journal of Botany·Linda E GrahamJames M Graham
Oct 20, 2010·Plant, Cell & Environment·Guillaume TcherkezAgnes Grapin
Jan 20, 2010·Plant, Cell & Environment·Guillaume TcherkezJaleh Ghashghaie
Jun 1, 2012·Plant, Cell & Environment·Guillaume TcherkezRichard Bligny
Sep 17, 2014·Plant, Cell & Environment·Susanne von Caemmerer, John R Evans
Dec 1, 2007·Functional Plant Biology : FPB·Guillaume Tcherkez, Graham D Farquhar
May 1, 2008·Functional Plant Biology : FPB·Guillaume Tcherkez, Graham D Farquhar
Aug 14, 2012·Plant, Cell & Environment·John R Evans, Susanne von Caemmerer
Oct 17, 2008·Plant Physiology·Gary J LaniganUlli Seibt
Oct 15, 2011·The Journal of Physical Chemistry. B·Anastassia N Alexandrova, William L Jorgensen

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