Vif-deficient HIV reverse transcription complexes (RTCs) are subject to structural changes and mutation of RTC-associated reverse transcription products

Virology
J M CarrP Li

Abstract

Reverse transcription (RTn) in HIV-infected cells occurs in a nucleoprotein complex termed the reverse transcription complex (RTC). RTCs containing RT activity and integrase (IN) were shown to be heterogeneous in size and density on sucrose velocity and equilibrium gradients. WT and Vif-deficient (Deltavif) RTCs produced by infection with virus from permissive cells displayed similar sedimentation characteristics, while RTCs from Deltavif virus produced in non-permissive cells demonstrated a reduction in the major RTC form and more of the RTn products in rapidly sedimenting structures. APOBEC3G derived from virions did not co-sediment with RTCs, but RTCs from Deltavif infections showed elevated levels of mutations in RTn products, consistent with APOBEC3G and other mutational mechanisms. The most mutated transcripts were present within rapidly sedimenting RTCs. Thus, virus without functional vif, produced from non-permissive cells, forms abnormal RTCs that contain increased mutation of RTC-associated RTn products in newly infected target cells.

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Citations

Jun 26, 2012·Virus Research·Julien BatisseJean-Christophe Paillart
Jul 19, 2011·Journal of Molecular Biology·Elena Britan-RosichMoshe Kotler
Mar 1, 2008·Virology·Jenny L Anderson, Thomas J Hope
Jul 1, 2016·Microbiology and Molecular Biology Reviews : MMBR·Guangdi Li, Erik De Clercq

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