Vigilin contains a functional nuclear localisation sequence and is present in both the cytoplasm and the nucleus

FEBS Letters
S KüglerC Kruse

Abstract

Vigilin is a member of the KH protein family and contains 14 tandemly arranged potential RNA-binding domains. Between KH domains 2 and 3 we have identified a nuclear localization sequence by cloning this sequence into the NH2-terminal region of phage T7 RNA polymerase as a reporter protein and by showing its transfer into the nucleus. Furthermore we provide experimental evidence that Vigilin is present both in the nucleus and in the cytoplasm in similar concentrations. These observations support the notion that Vigilin may shuttle between nucleus and cytoplasm presumably in contact with RNA molecules.

References

May 1, 1990·Molecular and Cellular Biology·J Engebrecht, G S Roeder
Aug 1, 1995·FEBS Letters·G Simos, E C Hurt
Jan 23, 1995·FEBS Letters·M A Castiglone MorelliG Travè
Sep 1, 1994·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H Tamaki, S Yamashina
Jan 1, 1994·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·M Klinger
Aug 13, 1993·Cell·M S Schmidt-ZachmannE A Nigg
Aug 27, 1993·Cell·R A Laskey, C Dingwall
Mar 11, 1993·Nucleic Acids Research·H SiomiG Dreyfuss

❮ Previous
Next ❯

Citations

Jul 9, 1999·Biopolymers·S AdinolfiA Pastore
Apr 28, 2004·The International Journal of Biochemistry & Cell Biology·Tillman VollbrandtCharli Kruse
Feb 5, 2000·The Biochemical Journal·C KruseP K Müller
Sep 10, 2004·Nucleic Acids Research·Ai-min LiJudith L Fridovich-Keil
Jul 18, 2002·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·I HilgendorfC Kruse
Aug 2, 2003·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Inken HilgendorfCharli Kruse
May 23, 2012·Proteome Science·Stephen Hsueh-Jeng LuPeter St George-Hyslop
Aug 1, 1996·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·E RumpelW Kühnel
Jun 25, 1998·Molecular and Cellular Biology·H KanamoriD J Shapiro
May 20, 1999·American Journal of Medical Genetics·J R Currie, W T Brown
Apr 25, 2006·The Journal of Biological Chemistry·Francesc-Xavier MarsellachFernando Azorín
Jun 3, 2015·Cell Biology International·Ling WeiYang Qin
Oct 5, 2017·Wiley Interdisciplinary Reviews. RNA·Matthew Hk Cheng, Ralf-Peter Jansen
Jun 25, 2019·Experimental Cell Research·Wei ZhouXiaohong Fang
May 5, 2021·Molecular and Cellular Biology·Shahid BandayMohammad Altaf
Nov 3, 2010·Biochimica Et Biophysica Acta·Marta BatlleFernando Azorín

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.