Abstract
Hypercholesterolemia is one of the causes of female infertility, and as a common fatty acid in follicular fluid, palmitic acid (PA) level plays a vital role in granule cell which is closely related to the developmental potential of follicle. The ovarian granulosa cell-like human granulosa (KGN) cell line and the immortalized normal ovarian surface epithelial cell line (IOSE80) were used to verify the effect of PA on cell viability and apoptosis by MTT and flow cytometry assay, respectively. Then mitochondria damage was confirmed by mitochondrial membrane potential, mitochondrial ROS detection assay and western blot in KGN cells. Thorough luciferase reporter assay and RIP-qPCR, the relationship between vigilin and ER-β was investigated. In our study, PA induced mitochondrial damage-mediated cell apoptosis of KGN cells was dose-dependently, while PA shown no effects on in IOSE80 cells. Then the role of calcineurin (CnA)-mediated Drp1 signaling pathway on KGN cells was confirmed by treating with Mdivi-1 or FK506T. In addition, the changed level of vigilin and ER-β was observed in cell apoptosis of KGN cells induced by PA. By transfecting vigilin vector or ER-β vector into KGN cells, respectively, vigilin and ER-β were demonstrated...Continue Reading
References
Apr 21, 1986·FEBS Letters·N H Fidge
Aug 1, 1996·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·E RumpelW Kühnel
Dec 31, 1997·Arteriosclerosis, Thrombosis, and Vascular Biology·D S ChiuK D O'Brien
Jul 19, 2001·Endocrinology·Y M MuH Nawata
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 25, 2004·Molecular Endocrinology·Simon ChuPeter J Fuller
May 12, 2005·Animal Reproduction Science·T VanholderA de Kruif
Jan 10, 2006·Cancer Letters·Gwendal Lazennec
Jun 13, 2006·Climacteric : the Journal of the International Menopause Society·J A Gustafsson
Apr 5, 2007·Nature Protocols·Tiina PeritzJames Eberwine
Oct 11, 2008·Journal of Cellular Biochemistry·Carolyn M Klinge
May 25, 2010·Biochemical and Biophysical Research Communications·Margaret Warner, Jan-Ake Gustafsson
Oct 1, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Willard W SharpStephen L Archer
Apr 15, 2014·The FEBS Journal·Qiuying LiuYang Qin
May 23, 2014·The Journal of Clinical Endocrinology and Metabolism·Enrique F SchistermanGermaine M Buck Louis
Sep 17, 2014·Reproductive Biology and Endocrinology : RB&E·Fahimeh Ramezani TehraniFereidoun Azizi
Sep 30, 2014·Current Biology : CB·Osamu UdagawaNaotada Ishihara
Nov 20, 2015·Cell Death & Disease·S XuK-S Park
Sep 27, 2016·Nature Communications·Mehrpouya B MobinMarkus Stoffel
Feb 10, 2017·Journal of Cellular Biochemistry·Zeting YuanWen Peng
May 4, 2017·Immunological Reviews·Bart Tummers, Douglas R Green
Aug 26, 2017·International Journal of Molecular Sciences·Ye YangBoyang Yu
Oct 7, 2018·Translational Oncology·Ho-Hyung WooSetsuko K Chambers
Jan 20, 2019·Brain Research·Xiangyang YuHongyin Du