Vigilin interacts with ER-β to protect against palmitic acid-induced granulosa cells apoptosis via inhibiting calcineurin-mediated Drp1 signaling pathway.

Steroids
Hong-Qing LiaoJing Zhou

Abstract

Hypercholesterolemia is one of the causes of female infertility, and as a common fatty acid in follicular fluid, palmitic acid (PA) level plays a vital role in granule cell which is closely related to the developmental potential of follicle. The ovarian granulosa cell-like human granulosa (KGN) cell line and the immortalized normal ovarian surface epithelial cell line (IOSE80) were used to verify the effect of PA on cell viability and apoptosis by MTT and flow cytometry assay, respectively. Then mitochondria damage was confirmed by mitochondrial membrane potential, mitochondrial ROS detection assay and western blot in KGN cells. Thorough luciferase reporter assay and RIP-qPCR, the relationship between vigilin and ER-β was investigated. In our study, PA induced mitochondrial damage-mediated cell apoptosis of KGN cells was dose-dependently, while PA shown no effects on in IOSE80 cells. Then the role of calcineurin (CnA)-mediated Drp1 signaling pathway on KGN cells was confirmed by treating with Mdivi-1 or FK506T. In addition, the changed level of vigilin and ER-β was observed in cell apoptosis of KGN cells induced by PA. By transfecting vigilin vector or ER-β vector into KGN cells, respectively, vigilin and ER-β were demonstrated...Continue Reading

References

Aug 1, 1996·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·E RumpelW Kühnel
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 13, 2006·Climacteric : the Journal of the International Menopause Society·J A Gustafsson
Apr 5, 2007·Nature Protocols·Tiina PeritzJames Eberwine
Oct 11, 2008·Journal of Cellular Biochemistry·Carolyn M Klinge
Oct 1, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Willard W SharpStephen L Archer
May 23, 2014·The Journal of Clinical Endocrinology and Metabolism·Enrique F SchistermanGermaine M Buck Louis
Sep 17, 2014·Reproductive Biology and Endocrinology : RB&E·Fahimeh Ramezani TehraniFereidoun Azizi
Sep 27, 2016·Nature Communications·Mehrpouya B MobinMarkus Stoffel
May 4, 2017·Immunological Reviews·Bart Tummers, Douglas R Green

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft
E RumpelW Kühnel
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
Ling WeiYang Qin
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
Wenli YangYang Qin
© 2021 Meta ULC. All rights reserved