VIGS vectors for gene silencing: many targets, many tools

Annual Review of Plant Biology
Dominique Robertson

Abstract

The discovery that plants recognize and degrade invading viral RNA caused a paradigm shift in our understanding of viral/host interactions. Combined with the discovery that plants cosuppress their own genes if they are transformed with homologous transgenes, new models for both plant intercellular communication and viral defense have emerged. Plant biologists adapted homology-based defense mechanisms triggered by incoming viruses to target individual genes for silencing in a process called virus-induced gene silencing (VIGS). Both VIGS- and dsRNA-containing transformation cassettes are increasingly being used for reverse genetics as part of an integrated approach to determining gene function. Virus-derived vectors silence gene expression without transformation and selection. However, because viruses also alter gene expression in their host, the process of VIGS must be understood. This review examines how DNA and RNA viruses have been modified to silence plant gene expression. I discuss advantages and disadvantages of VIGS in determining gene function and guidelines for the safe use of viral vectors.

References

Jun 1, 1973·The Journal of General Virology·F Brown, R Hull
Aug 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R T FraleyS C Woo
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·M H KumagaiL K Grill
Jan 1, 1995·Trends in Genetics : TIG·M A Matzke, A J Matzke
Sep 1, 1994·Molecular Plant-microbe Interactions : MPMI·W G DoughertyW M Proebsting
Mar 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·R HöfgenD von Wettstein
Feb 11, 1994·Cell·M WasseneggerH L Sänger
Oct 23, 1997·Nature·O Voinnet, D C Baulcombe
Feb 12, 1998·The Plant Journal : for Cell and Molecular Biology·K J OparkaR N Beachy
Jun 9, 1998·Plant Molecular Biology·M Wassenegger, T Pélissier
Oct 1, 1996·Nature Biotechnology·M Tepfer, M Jacquemond
Jun 23, 1998·The Plant Cell·M T RuizD C Baulcombe
Jul 24, 1998·Trends in Genetics : TIG·M K Montgomery, A Fire
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·R AnandalakshmiV B Vance
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·P M WaterhouseM B Wang
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·A HanssonM Hansson
Jul 13, 1999·The Plant Cell·F G RatcliffD C Baulcombe
Nov 26, 1999·The Plant Journal : for Cell and Molecular Biology·S M Angell, D C Baulcombe
Jan 29, 2000·Virology·S A MacFarlane, A H Popovich
Feb 11, 2000·RNA·T Pélissier, M Wassenegger

❮ Previous
Next ❯

Citations

Jun 7, 2012·Protoplasma·Pranav Pankaj SahuManoj Prasad
Nov 27, 2010·Journal of Experimental Botany·Lynette Brownfield, Claudia Köhler
Aug 11, 2012·Molecular Plant-microbe Interactions : MPMI·Aureliano BombarelyGregory B Martin
Oct 9, 2007·Molecular Plant-microbe Interactions : MPMI·Boovaraghan BalajiJames Schoelz
Jul 12, 2008·Molecular Plant-microbe Interactions : MPMI·Michael M GoodinSteven A Lommel
Oct 24, 2008·Phytopathology·Jimena Carrillo-TrippRafael F Rivera-Bustamante
Dec 14, 2004·Plant Physiology·Tracy ValentineChristophe Lacomme
Dec 26, 2006·Plant Physiology·Hsiang-Chia LuHsin-Hung Yeh
Dec 7, 2007·Plant Physiology·Mansour KarimiPierre Hilson
Jan 8, 2009·Plant Physiology·Steven R Scofield, Richard S Nelson
Jan 8, 2009·Plant Physiology·Andrew H PatersonXiyin Wang
Jan 9, 2009·Plant Physiology·Fabián E Vaistij, Louise Jones
Apr 15, 2010·Plant Physiology·Yang TangYule Liu
Feb 7, 2008·Annual Review of Plant Biology·Jörg Ziegler, Peter J Facchini
May 18, 2007·Annual Review of Phytopathology·Andrew F Bent, David Mackey
Aug 3, 2012·Plant Methods·John Richard TuttleDominique Robertson
Jul 9, 2013·BMC Research Notes·Pramod K KandothMelissa G Mitchum
Apr 15, 2011·Uirusu·Noriko Yamagishi, Nobuyuki Yoshikawa

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
PCR

Software Mentioned

VIGS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Plant Journal : for Cell and Molecular Biology
Tessa M Burch-SmithS P Dinesh-Kumar
Methods : a Companion to Methods in Enzymology
Rui LuDavid Baulcombe
The Plant Journal : for Cell and Molecular Biology
Yule LiuS P Dinesh-Kumar
The Plant Journal : for Cell and Molecular Biology
Gianinna BrignetiJonathan D G Jones
© 2021 Meta ULC. All rights reserved