Vimentin regulates activation of the NLRP3 inflammasome

Nature Communications
Gimena dos SantosKaren M Ridge

Abstract

Activation of the NLRP3 inflammasome and subsequent maturation of IL-1β have been implicated in acute lung injury (ALI), resulting in inflammation and fibrosis. We investigated the role of vimentin, a type III intermediate filament, in this process using three well-characterized murine models of ALI known to require NLRP3 inflammasome activation. We demonstrate that central pathophysiologic events in ALI (inflammation, IL-1β levels, endothelial and alveolar epithelial barrier permeability, remodelling and fibrosis) are attenuated in the lungs of Vim(-/-) mice challenged with LPS, bleomycin and asbestos. Bone marrow chimeric mice lacking vimentin have reduced IL-1β levels and attenuated lung injury and fibrosis following bleomycin exposure. Furthermore, decreased active caspase-1 and IL-1β levels are observed in vitro in Vim(-/-) and vimentin-knockdown macrophages. Importantly, we show direct protein-protein interaction between NLRP3 and vimentin. This study provides insights into lung inflammation and fibrosis and suggests that vimentin may be a key regulator of the NLRP3 inflammasome.

References

Dec 1, 1989·The American Review of Respiratory Disease·R F JacobsG D Campbell
Jan 1, 1986·The Japanese Journal of Physiology·J P ButlerT Takishima
Jun 26, 2001·Cell Death and Differentiation·Y ByunV L Cryns
Aug 25, 2001·The New England Journal of Medicine·T J Gross, G W Hunninghake
Dec 17, 2002·Nature Cell Biology·Nirit Mor-VakninDavid M Markovitz
Jan 27, 2004·The American Journal of Pathology·David DinsdaleMarcus E Peter
Jan 30, 2004·Annual Review of Medicine·Victor J ThannickalFernando J Martinez
Jul 17, 2004·American Journal of Respiratory and Critical Care Medicine·Zaher S AzzamKaren M Ridge
Jul 30, 2005·Molecular Endocrinology·Grant D BarishRonald M Evans
Jan 13, 2006·Nature·Fabio MartinonJürg Tschopp
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·G R Scott BudingerNavdeep S Chandel
Aug 16, 2006·The New England Journal of Medicine·Ganesh SuntharalingamNicki Panoskaltsis
Oct 19, 2006·Journal of Molecular Biology·Eran PerlsonMike Fainzilber
Dec 21, 2006·Science's STKE : Signal Transduction Knowledge Environment·Hanna-Mari Pallari, John E Eriksson
May 22, 2007·Experimental Cell Research·Johanna IvaskaJohn E Eriksson
Jul 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Helena L WangG R Scott Budinger
Nov 6, 2007·Antioxidants & Redox Signaling·Rui-Ming Liu
Nov 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lloyd S MillerRobert L Modlin
Nov 13, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Bethany B Moore, Cory M Hogaboam
Apr 23, 2008·The Journal of Cell Biology·Dale K ShumakerRobert D Goldman
Jun 26, 2008·Proceedings of the National Academy of Sciences of the United States of America·Suzanne L CasselFayyaz S Sutterwala
Jan 6, 2009·Immunological Reviews·Mohamed Lamkanfi, Vishva M Dixit
Feb 17, 2009·American Journal of Respiratory and Critical Care Medicine·Pamela GasseIsabelle Couillin
Jul 9, 2009·Physiological Reviews·Débora S Faffe, Walter A Zin
Jul 10, 2009·The Journal of Clinical Investigation·M Bishr Omary
Jul 10, 2009·The Journal of Clinical Investigation·John E ErikssonRobert D Goldman
Jul 10, 2009·The Journal of Clinical Investigation·Harald HerrmannUeli Aebi
Jul 10, 2009·The Journal of Clinical Investigation·Ronald K H Liem, Albee Messing
Jul 10, 2009·The Journal of Clinical Investigation·Shuhua SongRoy A Quinlan
Dec 31, 2009·PLoS Pathogens·Mohamed Lamkanfi, Vishva M Dixit
Jan 6, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Stéphanie PortetHarald Herrmann
Jan 30, 2010·Nature·Daniel A Fletcher, R Dyche Mullins
Feb 13, 2010·Cellular Microbiology·Bibhuti B MishraElsa Anes
Feb 24, 2010·The Journal of Experimental Medicine·Mark S WilsonThomas A Wynn
Mar 23, 2010·Cell·Kate Schroder, Jurg Tschopp
Aug 25, 2010·The Journal of Cell Biology·Fei LiuDaniel J Tschumperlin
Dec 3, 2010·Nature·Rongbin ZhouJürg Tschopp
May 3, 2011·American Journal of Respiratory Cell and Molecular Biology·Gustavo Matute-BelloUNKNOWN Acute Lung Injury in Animals Study Group
May 13, 2011·Molecular Biology of the Cell·Oxana E NekrasovaAlexander A Minin
Jun 17, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Franz BauernfeindVeit Hornung
Jul 6, 2011·The Journal of Experimental Medicine·Thomas A Wynn
Jul 13, 2011·Molecular and Cellular Biology·Azariyas A Challa, Branko Stefanovic
Aug 2, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Micah R RogelKaren M Ridge
Jan 20, 2012·Nature·Till StrowigRichard Flavell

❮ Previous
Next ❯

Citations

Sep 16, 2015·International Journal of Molecular Sciences·Seok-Jo KimDavid W Kamp
Sep 30, 2015·American Journal of Respiratory Cell and Molecular Biology·Seonmin LeeAugustine M K Choi
Aug 31, 2016·Nature Microbiology·Brian C RussoMarcia B Goldberg
Oct 27, 2015·Frontiers in Immunology·Christopher N LaRock, Victor Nizet
Mar 24, 2016·Seminars in Immunopathology·L A Borthwick
Apr 21, 2016·Cells·Tim N Mak, Holger Brüggemann
Sep 20, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Lei JiangMingyan Zhao
Sep 22, 2016·Particle and Fibre Toxicology·Mutlay Sayan, Brooke T Mossman
Oct 27, 2016·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Yang ChenYang Zhang
Apr 30, 2016·Scientific Reports·Qiang XiaoWeiqiang Lu
Jun 27, 2017·PLoS Pathogens·Maria J Mazon-MoyaSerge Mostowy
Feb 16, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Bria M CoatesKaren M Ridge
Nov 22, 2017·Annals of the American Thoracic Society·Sunad RangarajanVictor J Thannickal
Sep 28, 2017·Development·Emilia PeuhuJohanna Ivaska
Apr 30, 2017·American Journal of Physiology. Lung Cellular and Molecular Physiology·Fu Jun LiVeena B Antony
Dec 3, 2017·Cold Spring Harbor Perspectives in Biology·Elly M Hol, Yassemi Capetanaki
May 31, 2018·British Journal of Pharmacology·Tara Elizabeth ScottAdrian J Hobbs
Mar 20, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Phillip MunsonArti Shukla
Sep 22, 2018·Human Molecular Genetics·Teija PaakkolaReetta Hinttala
Jun 4, 2016·Nature Communications·Swati BhattacharyyaJohn Varga
Jan 16, 2019·Journal of Leukocyte Biology·Hemant Joshi, Sharon Celeste Morley
Jan 18, 2019·American Journal of Physiology. Lung Cellular and Molecular Physiology·Wu-Jian XuYong Song
Feb 24, 2019·Proceedings of the National Academy of Sciences of the United States of America·Michael A DavisMichael Gale
Aug 22, 2015·Nature Reviews. Immunology·Serge Mostowy, Avinash R Shenoy
Apr 18, 2019·Biological Chemistry·Ulrika WilhelmssonMilos Pekny
Jul 12, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Neha MishraLukas Bossaller
Sep 2, 2017·American Journal of Physiology. Lung Cellular and Molecular Physiology·Dietmar SpenglerMartin F Krause
Oct 14, 2017·Bioscience Reports·Michael Kasper, Kathrin Barth
Mar 4, 2020·International Journal of Molecular Sciences·Soo Jung ChoJong-Seok Moon
Jul 8, 2020·International Journal of Molecular Sciences·Irene RamosDolores Pérez-Sala
Feb 3, 2018·International Journal of Molecular Medicine·Ju KuangXiaolin Wei
May 24, 2017·Proceedings of the National Academy of Sciences of the United States of America·Daniel AntfolkCecilia M Sahlgren
Jun 10, 2018·Nature Communications·Tali LangJames Harris
Dec 7, 2018·F1000Research·Rachel A BattagliaNatasha T Snider
Jul 12, 2019·Clinical & Translational Immunology·Helen I Warheit-NiemiBethany B Moore
Aug 29, 2019·Scientific Reports·Nicole C A van EngelandCecilia M Sahlgren

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
bronchoalveolar lavage
fluorescence-activated cell sorting
AFM
atomic force microscopy
PMA
Confocal microscopy
electrophoresis
bio-layer interferometry
biosensor

Software Mentioned

MATLAB
Fiji
Zeiss LSM Image
Cri NUANCE
GraphPad Prism
GraphPad
TissueGnostics
TissueFAXS
BLItz Pro

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved