Abstract
In astrocytes, the PGF(2alpha) or ionomycin treatment induces the phosphorylation at Ser38 and Ser82 of vimentin, a type III intermediate filament, by Ca(2+)/calmodulin-dependent protein kinase II (CaMKII). We found here that vimentin phospho-Ser82 was dephosphorylated much slower than phospho-Ser38. Vimentin phospho-Ser38 was dephosphorylated quickly by purified PP1 catalytic subunit (PP1c) in vitro, whereas phospho-Ser82 was insensitive to PP1c. Because PP1c directly bound to vimentin through a VxF motif (Val83-Asp84-Phe85), the PP1c active site appeared to be unable to approach phospho-Ser82, leading to the prolongation of the phosphorylation at Ser-82. In astrocytes, PP1calpha was in vivo associated with vimentin filaments. The repetitive treatment by ionomycin at a short interval resulted in the sustained elevation of Ser82 phosphorylation, leading to the marked disassembly of vimentin filaments. Taken together, these results suggest that vimentin is a novel member of binding partner of PP1c in astrocytes, and vimentin-Ser82 may act as a memory phosphorylation site.
References
Dec 1, 1990·Japanese Journal of Cancer Research : Gann·K SasakiM Nagao
Aug 13, 1987·Nature·M InagakiC Sato
Jun 1, 1982·Journal of Neuroimmunology·P A TrimmerL F Eng
Nov 1, 1995·The Journal of Cell Biology·M OgawaraH Sugiura
Oct 1, 1995·The Biochemical Journal·S Wera, B A Hemmings
May 14, 1993·Biochemical and Biophysical Research Communications·H ShimaM Nagao
Apr 15, 1997·The EMBO Journal·M P EgloffD Barford
Oct 6, 1997·The Journal of Biological Chemistry·N InagakiM Inagaki
Nov 27, 1999·The Journal of Biological Chemistry·H InadaM Inagaki
Mar 14, 2000·Current Biology : CB·S RaghavanL Alphey
Mar 27, 2003·The Journal of Biological Chemistry·Paulina WakulaMathieu Bollen
May 23, 2003·Glia·A Privat
May 28, 2004·Nature·Mohammed TerrakRoberto Dominguez
Feb 3, 2005·Journal of Pharmacological Sciences·Yoshitoku YoshidaYoshihisa Kudo
Sep 8, 2005·Current Opinion in Neurobiology·Nicola J Allen, Ben A Barres
Nov 2, 2005·The Journal of Cell Biology·Tomoya YamaguchiMasaki Inagaki
Jan 24, 2006·Nature Cell Biology·Mikko NieminenSirpa Jalkanen
Apr 1, 2006·Neuron Glia Biology·Jai-Yoon SulPhilip G Haydon
Citations
Sep 25, 2008·BMC Biochemistry·Bastienne BrauksiepeErwin R Schmidt
Feb 1, 2013·Molecular BioSystems·Monica MoniciAnna Caselli
May 27, 2008·FEBS Letters·Claire L HyderJohn E Eriksson
Dec 24, 2014·Journal of the American Chemical Society·Hui LiPeng-Ye Wang
Jun 15, 2010·Glia·Maja PotokarRobert Zorec
Mar 25, 2014·IUBMB Life·Hidemasa Goto, Masaki Inagaki
Oct 8, 2014·Reproductive Toxicology·Xi ZhangZhihong Cui
Jul 23, 2015·BMC Genomics·Tobias LautweinErwin R Schmidt
Apr 28, 2016·Cells·Azzurra Margiotta, Cecilia Bucci
May 2, 2019·EMBO Molecular Medicine·Ratnakar SinghFaye M Johnson
Jan 25, 2020·Molecular Cancer·Hongbao YangRui Chen
Jul 4, 2007·Genes & Development·Seyun Kim, Pierre A Coulombe