PMID: 9428694Jan 15, 1998Paper

Vinblastine-induced formation of tubulin polymers is electrostatically regulated and nucleated

European Journal of Biochemistry
S S Rai, J Wolff

Abstract

Vinblastine promotes tubulin polymerization as measured by turbidity at 400 nm. Unlike microtubule assembly, this mode of polymerization does not require GTP and, in fact, GTP inhibits polymerization, as do other G nucleotides in the potency order: GtetraP > or = GTP > GDP > GMP > no nucleotide. Inhibition is not nucleoside-specific as ATP, ADP, and CTP also inhibit, and inorganic oligophosphates are as inhibitory as nucleotides in the order tetraphosphate approximately triphosphate > pyrophosphate > phosphate. Inhibition of polymerization is a rough function of the number of anionic charges and can be mimicked by suramin or tartrate. It is not due to sequestration of magnesium or to debinding of vinblastine. The anion-induced decrease in turbidity generation is reflected in the amount of tubulin that is pelletable, but even in the absence of turbidity significant pelletable tubulin persists which can be assessed by 90 degree light scattering. Formation of this polymer is less sensitive to anions. Shearing of GTP-inhibited and vinblastine-induced samples promotes turbidity and addition of seeds made from vinblastine polymers leads to rapid increases in turbidity in a concentration-dependent manner. Adjustment of the vinblastine...Continue Reading

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Citations

May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·S S Rai, J Wolff
Aug 5, 2015·Biochimica Et Biophysica Acta·Caitlyn M RotondoStefan Siemann

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