VIP activates primordial follicles of rat through ERK-mTOR pathway in tissue culture

Reproduction : the Official Journal of the Society for the Study of Fertility
Song LiQingxue Zhang

Abstract

In vitro activation of primordial follicles is becoming more essential in assisted reproductive technologies. Vasoactive intestinal peptide (VIP) is one of the members of the neurotrophin family which has demonstrated to have an impact on follicle development in recent years. This study aims to investigate the effect of VIP on the activation of primordial follicles in neonatal rat in an in vitro culture system and to determine the relevant molecular mechanism of their activation. Ovaries of 4-day-old rats were examined for the expression of VIP receptors and were cultured in mediums containing VIP with or without inhibitors of the ERK-mTOR signalling pathway. They were then collected for histological analysis or measurement of the molecular expression of this pathway. The receptors of VIP were found in granular cells and oocytes of primordial and early-growing follicles in neonatal ovary. The ratio of growing follicle increased in the presence VIP at different concentrations, with the highest level of increase being observed in the 10-7 mol/L VIP-treated group. The ratio of PCNA-positive granular cells was also increased, while that of the apoptotic oocytes were decreased, and protein analysis showed increased phosphorylation o...Continue Reading

References

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Citations

Aug 8, 2020·Endocrine Reviews·Johanne GrosboisIsabelle Demeestere

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Methods Mentioned

BETA
gene-knockout
Protein Assay
PCR

Software Mentioned

SPSS Statistics
ImageJ

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