VIP and CRF reduce ADAMTS expression and function in osteoarthritis synovial fibroblasts

Journal of Cellular and Molecular Medicine
Selene Pérez-GarcíaYasmina Juarranz

Abstract

ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family is known to play an important role in the pathogenesis of osteoarthritis (OA), working on aggrecan degradation or altering the integrity of extracellular matrix (ECM). Thus, the main purpose of our study was to define the role of vasoactive intestinal peptide (VIP) and corticotrophin-releasing factor (CRF), as immunoregulatory neuropeptides, on ADAMTS production in synovial fibroblasts (SF) from OA patients and healthy donors (HD). OA- and HD-SF were stimulated with pro-inflammatory mediators and treated with VIP or CRF. Both neuropeptides decreased ADAMTS-4, -5, -7 and -12 expressions, aggrecanase activity, glycosaminoglycans (GAG), and cartilage oligomeric matrix protein (COMP) degradation after stimulation with fibronectin fragments (Fn-fs) in OA-SF. After stimulation with interleukin-1β, VIP reduced ADAMTS-4 and -5, and both neuropeptides decreased ADAMTS-7 production and COMP degradation. Moreover, VIP and CRF reduced Runx2 and β-catenin activation in OA-SF. Our data suggest that the role of VIP and CRF on ADAMTS expression and cartilage degradation could be related to the OA pathology since scarce effects were produced in HD-SF. In addition, th...Continue Reading

References

Mar 7, 2001·European Journal of Biochemistry·M N VankemmelbekeD J Buttle
Jan 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yuji YamanishiGary S Firestein
Jul 27, 2005·The Journal of Biological Chemistry·Tripti GaurJane B Lian
May 9, 2006·Biochemical and Biophysical Research Communications·Kannan ThirunavukkarasuSrinivasan Chandrasekhar
Aug 5, 2006·Annals of the New York Academy of Sciences·R P GomarizC Martinez
Jan 11, 2007·Molecular Biology Reports·Kannan ThirunavukkarasuTao Wei
Apr 25, 2007·Cellular and Molecular Life Sciences : CMLS·C TsatsanisA N Margioris
Nov 16, 2007·Arthritis Research & Therapy·Yvonne RengelSteffen Gay
Jan 30, 2008·Laboratory Investigation; a Journal of Technical Methods and Pathology·Takahito YuasaMotomi Enomoto-Iwamoto
Sep 30, 2008·Nature Clinical Practice. Rheumatology·Maripat Corr
Dec 20, 2008·The Journal of International Medical Research·K Huang, L D Wu
Jan 13, 2009·Bone·Frank P LuytenRik J Lories
Nov 13, 2009·Annals of the New York Academy of Sciences·Richard L HaugerFrank M Dautzenberg
Dec 25, 2009·Current Pharmaceutical Design·R P GomarizC Martínez
Jan 5, 2011·Protein & Cell·Edward A Lin, Chuan-Ju Liu
Jul 23, 2011·Biochimica Et Biophysica Acta·Linda Troeberg, Hideaki Nagase
Dec 20, 2011·Osteoarthritis and Cartilage·P M van der Kraan, W B van den Berg
Jan 17, 2012·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Qiang ZhangYan Wang
Mar 7, 2012·Arthritis and Rheumatism·Richard F LoeserMary B Goldring
Jan 4, 2013·Cold Spring Harbor Perspectives in Biology·Fanxin Long, David M Ornitz
Feb 2, 2013·Clinical Rheumatology·Enshui ZhangZhen Yuan
Aug 10, 2013·Annals of the Rheumatic Diseases·Yongjie LaiChuan-Ju Liu
Jan 11, 2014·PloS One·Carmen MartínezIsidoro González-Álvaro
Mar 13, 2014·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Lei DingJames A Martin
May 20, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Anna MeroGianfranco Pasut
Feb 24, 2015·Peptides·Tamás JuhászRóza Zákány

❮ Previous
Next ❯

Citations

Aug 16, 2016·Annals of the New York Academy of Sciences·Weiwei ZhaoDi Chen
Jan 27, 2018·International Journal of Molecular Sciences·Susanne Grässel, Dominique Muschter
Apr 18, 2018·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Shabnam Hemmati-SadeghiTilo Dehne
Dec 22, 2019·International Journal of Molecular Sciences·Carmen MartínezRosa P Gomariz
May 4, 2020·International Immunopharmacology·Zijian YanXiaoyun Pan

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsy
biopsies
PCR
ELISA
total hip arthroplasty
Assay

Software Mentioned

GraphPad
GraphPad Prism

Related Concepts

Related Feeds

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Related Papers

The Journal of International Medical Research
K Huang, L D Wu
Protein & Cell
Edward A Lin, Chuan-Ju Liu
Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte
Rebecca C SalterDipak P Ramji
© 2021 Meta ULC. All rights reserved