VIP and PACAP analogs regulate therapeutic targets in high-risk neuroblastoma cells

Peptides
Madryssa de BoisvilliersCorinne Chadéneau

Abstract

Neuroblastoma (NB) is a pediatric cancer. New therapies for high-risk NB aim to induce cell differentiation and to inhibit MYCN and ALK signaling in NB. The vasoactive intestinal peptide (VIP) and the pituitary adenylate cyclase-activating polypeptide (PACAP) are 2 related neuropeptides sharing common receptors. The level of VIP increases with NB differentiation. Here, the effects of VIP and PACAP analogs developed for therapeutic use were studied in MYCN-amplified NB SK-N-DZ and IMR-32 cells and in Kelly cells that in addition present the F1174L ALK mutation. As previously reported by our group in IMR-32 cells, VIP induced neuritogenesis in SK-N-DZ and Kelly cells and reduced MYCN expression in Kelly but not in SK-N-DZ cells. VIP decreased AKT activity in the ALK-mutated Kelly cells. These effects were PKA-dependent. IMR-32, SK-NDZ and Kelly cells expressed the genes encoding the 3 subtypes of VIP and PACAP receptors, VPAC1, VPAC2 and PAC1. In parallel to its effect on MYCN expression, VIP inhibited invasion in IMR-32 and Kelly cells. Among the 3 PACAP analogs tested, [Hyp(2)]PACAP-27 showed higher efficiency than VIP in Kelly cells. These results indicate that VIP and PACAP analogs act on molecular and cellular processes that...Continue Reading

References

Feb 18, 1992·Regulatory Peptides·M S O'DorisioT M O'Dorisio
Apr 1, 1995·Molecular Neurobiology·J M MullerA C Meunier
Jan 1, 1995·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·S FuldaF Berthold
Jan 1, 1993·Journal of Molecular Neuroscience : MN·I Gozes, D E Brenneman
Jun 1, 1993·Journal of Pediatric Surgery·Y ZaizenS Suita
Jun 2, 1997·The EMBO Journal·W A WeissJ M Bishop
Feb 3, 1999·Annals of the New York Academy of Sciences·M C FrühwaldT M O'Dorisio
Jan 4, 2001·American Journal of Surgery·J L Grosfeld
Feb 7, 2003·Current Pharmaceutical Design·Terry W MoodyRobert T Jensen
Mar 4, 2003·Nature Reviews. Cancer·Garrett M Brodeur
Sep 18, 2003·Journal of the National Cancer Institute·Catherine A BurkhartMichelle Haber
Sep 23, 2006·Regulatory Peptides·Jean-Marc MullerCorinne Chadéneau
Oct 24, 2006·Biochemical and Biophysical Research Communications·Jung-Hee KangDai H Chung
Nov 14, 2006·Current Opinion in Pediatrics·Matthew R Grimmer, William A Weiss
Nov 3, 2007·Surgical Oncology·Titilope A Ishola, Dai H Chung
Mar 21, 2008·Peptides·Steve BourgaultAlain Fournier
Sep 11, 2008·Proceedings of the National Academy of Sciences of the United States of America·Erik FredlundSven Påhlman
Sep 27, 2008·Cell Cycle·Meng QiaoArthur B Pardee
Dec 27, 2008·Pharmacology & Therapeutics·Louise Dickson, Keith Finlayson
Feb 4, 2009·The Prostate·Ana B Fernández-MartínezMaría J Carmena
Mar 17, 2010·Cancer Treatment Reviews·Shakeel Modak, Nai-Kong V Cheung
Aug 20, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Sara De BrouwerFrank Speleman
Aug 19, 2011·Seminars in Cancer Biology·Ulrica K WestermarkMarie Arsenian Henriksson
Mar 24, 2012·Cancer Cell·Shizhen ZhuA Thomas Look
May 23, 2013·Expert Opinion on Emerging Drugs·Victoria CastelPablo Berlanga
Aug 24, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Giuseppe BaroneLouis Chesler
Oct 3, 2013·Cold Spring Harbor Perspectives in Medicine·Miller Huang, William A Weiss
Mar 5, 2014·Molecular Cancer Research : MCR·Himisha Beltran
Mar 7, 2014·Nature Reviews. Cancer·Glenn M MarshallWilliam A Weiss
Oct 5, 2014·Journal of Molecular Neuroscience : MN·Souheyla BensalmaJean Marc Muller

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