VIP stimulation of cAMP production in corneal endothelial cells in tissue and organ cultures

Cornea
Shay-Whey M Koh, Beatrice Y J T Yue

Abstract

To demonstrate that vasoactive intestinal peptide (VIP), an immunosuppressive factor found in the aqueous humor, is a modulator of the corneal endothelium (CE) stimulating its intracellular cAMP production. Rabbit CE cells in cell culture and CE cells in cornea cup organ cultures established from bovine and human donor eyes were treated with VIP at varying concentrations (0, 10(-11)-10(-6) mol/L) for a constant time (4 minutes) or varying times (1, 3.25, 10, 15 minutes) at a constant concentration (1 x 10(-6) mol/L). Intracellular cAMP was extracted and its concentrations were determined by radioimmunoassay. Agonists that are known to modulate the intracellular cAMP concentrations of target cells were allowed to react with cultured rabbit CE cells at 1 x 10(-6) mol/L for 4 minutes. Vasoactive intestinal peptide stimulated the intracellular cAMP production in CE cells in a dose- and time-dependent manner. At concentrations lower than 10(-9) mol/L, VIP showed little effect. Treatment with 10(-8), 10(-7), and 10(-6) mol/L VIP for 4 minutes, however, increased the intracellular cAMP by 5.7-, 12.3-, and 9.5-fold, respectively, compared with the basal level in rabbit CE cell cultures, and by 19.5-, 38.7-, and 23.3-fold, respectively,...Continue Reading

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Citations

Dec 3, 2010·American Journal of Physiology. Cell Physiology·Shimin LiJoseph A Bonanno
Apr 5, 2005·Current Eye Research·Shay-Whey KohEve Higginbotham
Jul 29, 2006·Brain Research Reviews·Josef TrogerEduard Schmid
Mar 19, 2015·American Journal of Ophthalmology·Ahmad KheirkhahReza Dana
Jun 28, 2016·Biochemical and Biophysical Research Communications·Xing-Hua YuanDong Hun Lee
Jan 10, 2003·American Journal of Physiology. Cell Physiology·Xing Cai SunJoseph A Bonanno
Aug 12, 2018·The American Journal of Pathology·Vannarut SatitpitakulReza Dana

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