Abstract
Angiogenesis is a complex process consisting of the proliferation, migration and differentiation of endothelial cells, and it is essential for the progression of malignant solid tumors. In this report, we examine the effects of disintegrins (e.g. rhodostomin and accutin) and glycoprotein-lb-binding proteins (e.g. agkistin) on each step in angiogenesis using in vitro and in vivo models. Rhodostomin (but not agkistin) inhibited the viability of human umbilical vein endothelial cells (HUVECs) and capillary tube formation of HUVECs. Rhodostomin also inhibited HUVEC migration and invasion evoked by basic fibroblast growth factor (bFGF). In in vivo studies, rhodostomin inhibited bFGF-, but not vascular-endothelial-growth-factor (VEGF)- associated angiogenesis in chick chorioallantoic membrane model, blocked both bFGF and B16F10 melanoma cell-induced neovascularization, and suppressed the growth of subcutaneously inoculated B16F10 solid tumor, leading to a prolonged survival of the C57BL/6 mice treated with rhodostomin. The antiangiogenic effects of rhodostomin on bFGF-treated HUVECs may be mainly related to the blockade of the interaction of endothelial alpha(v)beta(3) and extracellular matrix.
Citations
Jul 3, 2013·The International Journal of Biochemistry & Cell Biology·Cezary Marcinkiewicz
Jun 10, 2010·PloS One·Radu O MineaFrancis S Markland
Apr 1, 2014·BioMed Research International·Leonardo A CalderonAndreimar M Soares
Jan 30, 2016·Molecular Carcinogenesis·Ons ZakraouiKhadija Essafi-Benkhadir
Mar 1, 2011·Toxicon : Official Journal of the International Society on Toxinology·Radu MineaFrancis Markland
Feb 24, 2007·Toxicon : Official Journal of the International Society on Toxinology·Jing TianMary Ann McLane
Aug 23, 2006·Journal of Cellular Biochemistry·Aixia RenYaozhou Zhang
Aug 15, 2015·Toxicon : Official Journal of the International Society on Toxinology·I Lima-Dos-SantosA M Moura-da-Silva
Feb 22, 2011·Toxicon : Official Journal of the International Society on Toxinology·Sara LucenaJohn C Perez
Jul 28, 2004·Biochimica Et Biophysica Acta·Elena AlimentiNorma Staiano
Nov 26, 2013·Toxicon : Official Journal of the International Society on Toxinology·Sara E LucenaElda E Sánchez
Sep 10, 2014·International Journal of Biological Macromolecules·David Collares AchêVeridiana de Melo Rodrigues
Jun 15, 2016·Toxins·Ana M Moura-da-SilvaRichard H Valente
Mar 12, 2004·Critical Reviews in Oncology/hematology·Alessandro MorabitoGiampietro Gasparini