Viperatoxin-II: A novel viper venom protein as an effective bactericidal agent

FEBS Open Bio
Ramar Perumal SamyGautam Sethi

Abstract

Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) have become a rising threat to public health. There is an urgent need for development of promising new therapeutic agents against drug resistant bacteria like S. aureus. This report discusses purification and characterization of proteins from Indian Russell's viper snake venom. Novel 15-kDa proteins called "Viperatoxin" (VipTx-I and VipTx-II) were extracted from the whole venom and evaluated using in vitro antimicrobial experiments. The N-terminal amino acid sequence of "Viperatoxin" showed high sequence homology to daboiatoxin isolated from the same venom and also matched phospholipase A2 (PLA2) enzymes isolated from other snake venoms. In an in vitro plate assay, VipTx-II but not VipTx-I showed strong antimicrobial effects against S. aureus and Burkholderia pseudomallei (KHW & TES), Proteus vulgaris and P. mirabilis. The VipTx-II was further tested by a broth-dilution assay at 100-3.1 μg/ml concentrations. The most potent bactericidal effect was found at the lowest dilutions (MICs of 6.25 μg/ml) against B. pseudomallei, S. aureus and P. vulgaris (MICs of 12.25 μg/ml). Electron microscopic investigation revealed that the protein-induced bactericidal potenc...Continue Reading

References

Jan 1, 1991·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·C K WatsonA R Pursell
Nov 7, 1998·Biochimica Et Biophysica Acta·K Matsuzaki
Jun 6, 2000·American Journal of Physiology. Cell Physiology·J I Kourie, A A Shorthouse
Dec 15, 2000·Journal of Molecular Biology·P V DubovskiiA S Arseniev
Feb 27, 2003·Antimicrobial Agents and Chemotherapy·Hongxia Zhao, Paavo K J Kinnunen
May 28, 2003·Lancet·N J White
Jul 24, 2003·Toxicon : Official Journal of the International Society on Toxinology·Marcos Hikari ToyamaSergio Marangoni
Mar 17, 2004·Toxicon : Official Journal of the International Society on Toxinology·Andreimar M Soares, José R Giglio
Mar 17, 2004·Toxicon : Official Journal of the International Society on Toxinology·Bruno LomonteLeonel Calderón
Jul 1, 2004·Toxicon : Official Journal of the International Society on Toxinology·Vitelbina NúñezBruno Lomonte
Jul 29, 2004·Current Medicinal Chemistry·Christina R Harris, Atli Thorarensen
Aug 11, 2004·Toxicon : Official Journal of the International Society on Toxinology·Veridiana M RodriguesAndreimar M Soares
Jan 22, 2005·Protein Science : a Publication of the Protein Society·Garima SinghTej P Singh
Feb 11, 2005·Nature Reviews. Microbiology·Kim A Brogden
Apr 9, 2005·The New England Journal of Medicine·Loren G MillerBrad Spellberg
Apr 21, 2005·Evidence-based Complementary and Alternative Medicine : ECAM·Deivy Clementino de LimaHelena Carla Castro
May 21, 2005·Toxicon : Official Journal of the International Society on Toxinology·Carlos SantamaríaBruno Lomonte
Mar 28, 2006·International Journal of Antimicrobial Agents·Sung-Tae YangJae Il Kim
Aug 18, 2006·The New England Journal of Medicine·Gregory J MoranUNKNOWN EMERGEncy ID Net Study Group
Nov 15, 2006·Cellular and Molecular Life Sciences : CMLS·D C I KohK Jeyaseelan
Sep 10, 2008·Trends in Biochemical Sciences·Gregor Anderluh, Jeremy H Lakey
Oct 29, 2008·Evidence-based Complementary and Alternative Medicine : ECAM·Bruno L FerreiraHelena C Castro
Jan 16, 2009·Transactions of the Royal Society of Tropical Medicine and Hygiene·Chiranjay MukhopadhyayIndira Bairy
Feb 1, 2011·Toxicon : Official Journal of the International Society on Toxinology·Tamara SajevicIgor Križaj
Feb 22, 2011·Toxicon : Official Journal of the International Society on Toxinology·Gargi MaityDebasish Bhattacharyya

❮ Previous
Next ❯

Citations

Jun 2, 2016·Applied Microbiology and Biotechnology·Yau Sang ChanWai Yee Chan
Sep 27, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ng Zhang Jin, Subash C B Gopinath
Dec 28, 2016·Toxins·Diana Aline Morais Ferreira NôgaAlessandra Mussi Ribeiro
Jan 25, 2020·PloS One·Justin L RheubertRobert A Charvat
Apr 25, 2020·Toxins·Clara Pérez-PeinadoDavid Andreu
Nov 17, 2020·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Kristina GopcevicMario Cindric
Aug 9, 2021·Trends in Parasitology·Samuel Cota TeixeiraVeridiana de Melo Rodrigues

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
electron microscopy
protein assay
ELISA
gel-filtration
transmission electron microscopy

Software Mentioned

Basic Local Alignment Search Tool ( BLAST )
Fit
BLAST
MS
ExPASy

Related Concepts

Related Feeds

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.